Egg ‐associated secretions from the brown planthopper (Nilaparvata lugens) activate rice immune responses

In this study, we found that both infestation of rice by gravid BPH female adults and treatment with the EAS elicited a strong and rapid accumulation of jasmonic acid (JA), JA-isoleucine, and hydrogen peroxide in rice. Proteome sequencing of EAS led to 110 proteins being identified and 55 proteins with 2 or more unique peptides. These findings point to novel proteins affecting interactions between planthoppers and rice during oviposition. AbstractThe brown planthopper (BPH,Nilaparvata lugens) is a notorious sap-sucking insect pest that damages rice (Oryza sativa) plants throughout Asia. During BPH feeding, saliva enters rice plant tissues, whereas during oviposition egg-associated secretions (EAS) are deposited in damaged plant tissue. Dynamic changes in rice to planthopper salivary effectors have been widely reported. However, the effects of EAS from planthopper on rice immunity remains largely unexplored. In this study, we found that both infestation of rice by gravid BPH female adults and treatment with the EAS elicited a strong and rapid accumulation of jasmonic acid (JA), JA-isoleucine, and hydrogen peroxide in rice. EAS enhanced plant defenses not only in rice but also in tobacco, and these impaired the performance of BPH on rice, as well as the performance of aphids and whiteflies on tobacco. High-throughput proteome sequencing of EAS led to 110 proteins being identified and 53 proteins with 2 or more unique peptides being detected. Some proteins from BPH EAS were also...

Source: Insect Science - November 27, 2023 Category: Biology Authors: Jing Li, Shuai Li, Jing Li, Xinyang Tan, Zhichang Zhao, Lei Jiang, Ary A. Hoffmann, Jichao Fang, Rui Ji Tags: ORIGINAL ARTICLE Source Type: research