< em > Propionibacterium freudenreichii < /em > CIRM-BIA 129 mitigates colitis through S layer protein B-dependent epithelial strengthening

Am J Physiol Gastrointest Liver Physiol. 2023 Nov 21. doi: 10.1152/ajpgi.00198.2023. Online ahead of print.ABSTRACTThe growing incidence of human diseases involving inflammation and increased gut permeability makes the quest for protective functional foods more crucial than ever. Propionibacterium freudenreichii (Pf) is a beneficial bacterium used in the dairy and probiotic industries. Selected strains exert anti-inflammatory effects, and the present work addresses whether the Pf CIRM-BIA129, consumed daily in a preventive way, could protect mice from acute colitis induced by dextran sodium sulfate (DSS), and more precisely whether it could protect from intestinal epithelial breakdown induced by inflammation. Pf CIRM-BIA129 mitigated colitis severity and inhibited DSS-induced permeability. It limited crypt length reduction and promoted the expression of Zonula Occludens-1 (ZO-1), without reducing interleukin-1beta mRNA (il-1ß) expression. In vitro, Pf CIRM-BIA129 prevented the disruption of a Caco-2 monolayer induced by pro-inflammatory cytokines. It increased transepithelial electrical resistance (TEER) and inhibited permeability induced by inflammation, along with an increased ZO-1 expression. Extracellular vesicles (EVs) from Pf CIRM-BIA129, carrying the surface layer protein (SlpB), reproduced the protective effect of Pf CIRM-BIA129. A mutant strain deleted for slpB (ΔslpB), or EVs from this mutant strain, had lost their protective effects, and worsened both DSS-induced...
Source: American Journal of Physiology. Gastrointestinal and Liver Physiology - Category: Physiology Authors: Source Type: research