Nephroprotective effect of PHYMIN-22 on ethylene glycol induced urolithiasis rat model

AbstractThe present study was designed to evaluate the antiurolithiatic effect of PHYMIN-22 against ethylene glycol ‐induced urolithiasis in rats. Healthy Albino male rats with 200–230 g body weight were randomly divided into five groups, each with 5 animals, control group, EG group (0.75%), PHYMIN-22 treatment group (0.75% EG 14 days and 100 mg/kg PHYMIN-22 next 14 days), PHYMIN-22 drug control group (10 0 mg/kg) and cystone treatment group (0.75% EG 14 days and 750 mg/kg cystone next 14 days). Biochemical testing was adopted for measuring the blood and urine parameters, as well as the level of antioxidants including superoxide dismutase (SOD), Catalase (Cat), Glutathione peroxidase (GPx) and gl utathione (GSH) in kidney tissues. Hematoxylin and eosin (HE) staining was utilized to observe the histopathological changes in the kidney tissue. End of the experiment the PHYMIN-22 treatment reduced the urine and serum calcium(p <  0.01;p <  0.01), oxalate (p <  0.01;p <  0.01), phosphate (p <  0.01;p <  0.01), uric acid (p <  0.001;p <  0.001), protein (p <  0.001;p <  0.001), and creatinine (p <  0.001;p <  0.001) respectively, serum indicators ALT (p <  0.001) and AST(p <  0.001) level and non-enzymic antioxidant GSH (p <  0.001) compared to EG induced urolithiasis animals (Diseased control group). PHYMIN-22 treatment significantly increased urine volume, pH, and ...
Source: Urolithiasis - Category: Urology & Nephrology Source Type: research