AAACH is a conserved motif in a cis-acting replication element that is artificially inserted into Senecavirus A genome

In this study, we constructed a set of SVA cDNA clones. Each of them contained one functionally inactivated Nc, and an extra SDM-modified Ac. Every cDNA clone had a unique SDM-modified Ac. The test of virus recovery showed that only two SVAs were rescued from their individual cDNA clones. They were AAACU- and AAACC-containing Ac genotypes. Both viruses were serially passaged in vitro for analyzing their viral characteristics. The results showed that both AAACU and AAACC genotypes were genetically stable during twenty passages, implying when the Nc was functionally inactivated, SVA could still use an AAACH-containing Ac to complete its own replication cycle.PMID:37952688 | DOI:10.1016/j.virusres.2023.199269

Source: Virus Research - November 12, 2023 Category: Virology Authors: Mengyao Wang Di Zhao Jing Li Lijie Zhu Xiaoxiao Duan Youming Zhang Yan Li Fuxiao Liu Source Type: research