The effect on m < sup > 6 < /sup > A methylation writer complex by the reduced MATR3 in pterygium

Acta Histochem. 2023 Oct 30;125(8):152101. doi: 10.1016/j.acthis.2023.152101. Online ahead of print.ABSTRACTPterygium is a common eye surface disease with high recurrence and unclear pathogenesis. In current study, RNA sequencing was conducted in 6 pairs of human pterygium and conjunctival tissues, and Matr3 as a novel candidate gene was significantly reduced in pterygium compared to control tissues. Moreover, immunoprecipitation was performed to pull down MATR3, and WTAP specially interacting with MATR3 in control but not pterygium was identified by mass spectrum. Immunoprecipitation was performed to validate the interaction between MATR3 and WTAP/METTL3/METTL14 complex. (Methylated) RNA immunoprecipitation was performed to further reveal that the binding affinity of WTAP and MATR3 was lost at 3' UTR of RNA molecules of down-regulated genes in pterygium. Overall, we figured out the loss of intercrossing between MATR3 and N6-methyladenosine methyltransferase complex, as well as indicated the potential impact on transcription of target genes in pterygium.PMID:37913560 | DOI:10.1016/j.acthis.2023.152101

Source: Acta Histochemica - November 1, 2023 Category: Biochemistry Authors: Qianqian Guo Shichao Han Source Type: research