Interleukin-6 reduces spheroid sizes of osteophytic cells derived from osteoarthritis knee joint via induction of apoptosis

Am J Pathol. 2023 Oct 31:S0002-9440(23)00411-X. doi: 10.1016/j.ajpath.2023.10.005. Online ahead of print.ABSTRACTOsteophyte in osteoarthritis (OA) joints contributes to restriction of joint movement, joint pain, and OA progression, but little is known about regulators of osteophyte. By examining the expression of the genes related to cartilage extracellular matrix (ECM), endochondral ossification and growth factor-signaling in articular cartilage and osteophyte obtained from OA knee joints, we demonstrated that several genes such as COL1A1, VCAN, BGLAP, BMP8B, RUNX2, and SOST are overexpressed in osteophyte compared to articular cartilage. Ratios of mesenchymal stem/progenitor cells (MSPCs), which were characterized by co-expression of CD105 and CD166, were significantly 2.2-fold higher in osteophytic cells than articular cells. Three-dimensional culture method for cartilage and osteophyte cells was developed by modification of cultures of self-assembled spheroid cell organoids (spheroids), and these spheroids cultured in the media for MSCs containing transforming growth factor-β3 showed characteristic morphologies and gene expression profiles of articular cartilage and osteophyte, respectively. We then studied the effects of interleukin-1β (IL-1β), tumor necrosis factor-α and IL-6 on the spheroids of articular and osteophytic cells, and provided, to the best of our knowledge, the first evidence that IL-6 suppresses spheroid size of osteophytic cells by inducing apoptosis...
Source: The American Journal of Pathology - Category: Pathology Authors: Source Type: research