Comprehensive characterization of pathogenic synovial fluid extracellular vesicles from knee osteoarthritis

Clin Immunol. 2023 Oct 20:109812. doi: 10.1016/j.clim.2023.109812. Online ahead of print.ABSTRACTSynovial fluid (SF) extracellular vesicles (EVs) play a pathogenic role in osteoarthritis (OA). However, the surface markers, cell and tissue origins, and effectors of these EVs are largely unknown. We found that SF EVs contained 692 peptides that were positively associated with knee radiographic OA severity; 57.4% of these pathogenic peptides were from 46 proteins of the immune system, predominantly the innate immune system. CSPG4, BGN, NRP1, and CD109 are the major surface markers of pathogenic SF EVs. Genes encoding surface marker CSPG4 and CD109 were highly expressed by chondrocytes from damaged cartilage, while VISG4, MARCO, CD163 and NRP1 were enriched in the synovial immune cells. The frequency of CSPG4+ and VSIG4+ EV subpopulations in OA SF was high. We conclude that pathogenic SF EVs carry knee OA severity-associated proteins and specific surface markers, which could be developed as a new source of diagnostic biomarkers or therapeutic targets in OA.PMID:37866785 | DOI:10.1016/j.clim.2023.109812

Source: Clinical Immunology - October 22, 2023 Category: Allergy & Immunology Authors: Xin Zhang Sisi Ma Syeda Iffat Naz Vaibhav Jain Erik J Soderblom Constantin Aliferis Virginia Byers Kraus Source Type: research