Effect of Biotin Starvation on Gene Expression in Komagataella phaffii Cells

In this study the effect of biotin starvation on gene expression inK.  phaffii at transcriptome level was investigated. It was demonstrated, that the response ofK.  phaffii cell to biotin deficiency strongly depends on the carbon source in the medium. In the media containing glycerol, biotin deficiency led to activation of the genes involved in biotin metabolism, glyoxylate cycle, and synthesis of acetyl-CoA in cytoplasm, as well as repression of the genes, involved in lipo- and gluconeogenesis. In the methanol-containing media, biotin deficiency primarily led to repression of the genes, involved in protein synthesis, and activation of cell response to oxidative stress.
Source: Biochemistry (Moscow) - Category: Biochemistry Source Type: research