Direct investigation of cell contraction signal networks by light-based perturbation methods

Pflugers Arch. 2023 Oct 18. doi: 10.1007/s00424-023-02864-2. Online ahead of print.ABSTRACTCell contraction plays an important role in many physiological and pathophysiological processes. This includes functions in skeletal, heart, and smooth muscle cells, which lead to highly coordinated contractions of multicellular assemblies, and functions in non-muscle cells, which are often highly localized in subcellular regions and transient in time. While the regulatory processes that control cell contraction in muscle cells are well understood, much less is known about cell contraction in non-muscle cells. In this review, we focus on the mechanisms that control cell contraction in space and time in non-muscle cells, and how they can be investigated by light-based methods. The review particularly focusses on signal networks and cytoskeletal components that together control subcellular contraction patterns to perform functions on the level of cells and tissues, such as directional migration and multicellular rearrangements during development. Key features of light-based methods that enable highly local and fast perturbations are highlighted, and how experimental strategies can capitalize on these features to uncover causal relationships in the complex signal networks that control cell contraction.PMID:37851146 | DOI:10.1007/s00424-023-02864-2

Source: Pflugers Archiv : European Journal of Physiology - October 18, 2023 Category: Physiology Authors: Perihan Nalbant Jessica Wagner Leif Dehmelt Source Type: research