Relationship between the Rod complex and peptidoglycan structure in Escherichia coli

The structures of the peptidoglycan of the wild-type strain, the mutant strain of the Rod complex, and its suppressor mutant strains were observed. The peptidoglycan purified from the Rod complex mutant had many large holes, whereas the number and size of holes were reduced in the suppressor mutants. We visualized the relationship between the Rod complex activity and the peptidoglycan structure. AbstractPeptidoglycan for elongation inEscherichia coli is synthesized by the Rod complex, which includes RodZ. Although various mutant strains of the Rod complex have been isolated, the relationship between the activity of the Rod complex and the overall physical and chemical structures of the peptidoglycan have not been reported. We constructed a RodZ mutant, termed RMR, and analyzed the growth rate, morphology, and other characteristics of cells producing the Rod complexes containing RMR. The growth and morphology of RMR cells were abnormal, and we isolated suppressor mutants from RMR cells. Most of the suppressor mutations were found in components of the Rod complex, suggesting that these suppressor mutations increase the integrity and/or the activity of the Rod complex. We purified peptidoglycan from wild-type, RMR, and suppressor mutant cells and observed their structures in detail. We found that the peptidoglycan purified from RMR cells had many large holes and different compositions of muropeptides from those of WT cells. The Rod complex may be a determinant not only for the w...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research