Investigating the Antimicrobial, Antioxidant, and Anticancer Effects of Elettaria cardamomum Seed Extract Conjugated to Green Synthesized Silver Nanoparticles by Laser Ablation

AbstractTo synthesize silver nanoparticles suspended in deionized water,Elettaria cardamomum (Car) seed extract and Car@Ag nanoparticles were prepared in three steps. The first used a hot aqueous extract ofElettaria cardamomum seed, and then, the second used pulsed laser ablation technique (PLAL) by Nd:YAG laser (λ = 1064 nm wavelength) at laser energy of 400 mJ, and the number of pulses was 500 pulses and third step for the synthesis of creating a non-conjugatedElettaria cardamomum extract. Ultraviolet visible (UV –Vis) spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) were used to investigate the optical, structural, and morphological characterizations of synthesized Car@AgNPs. The nanoparticles were spherical in shape, and a size distribut ion exhibited a mean size of 21 nm, according to the TEM findings. AgNPs and Car@AgNPs have free radical scavenging activity in the DPPH assay. Additionally, Car@AgNPs were examined in vitro for zones of inhibition against pathogenic bacteria likeEscherichia coli andStaphylococcus aureus. The inhibition rate ofEscherichia coli was higher than that observed inStaphylococcus aureus. Finally, Car@AgNPs demonstrated a potential cytotoxicity agent breast cancer (MCF-7) cell line that was evaluated by MTT assay.
Source: Plasmonics - Category: Biomedical Science Source Type: research