Genes, Vol. 14, Pages 1829: The Chloroplast Genome of Endive (Cichorium endivia L.): Cultivar Structural Variants and Transcriptome Responses to Stress Due to Rain Extreme Events

Genes, Vol. 14, Pages 1829: The Chloroplast Genome of Endive (Cichorium endivia L.): Cultivar Structural Variants and Transcriptome Responses to Stress Due to Rain Extreme Events
Genes doi: 10.3390/genes14091829
Authors:
Giulio Testone
Michele Lamprillo
Maria Gonnella
Giuseppe Arnesi
Anatoly Petrovich Sobolev
Riccardo Aiese Cigliano
Donato Giannino

The chloroplast (cp) genome diversity has been used in phylogeny studies, breeding, and variety protection, and its expression has been shown to play a role in stress response. Smooth- and curly-leafed endives (Cichorium endivia var. latifolium and var. crispum) are of nutritional and economic importance and are the target of ever-changing breeding programmes. A reference cp genome sequence was assembled and annotated (cultivar ‘Confiance’), which was 152,809 base pairs long, organized into the angiosperm-typical quadripartite structure, harboring two inverted repeats separated by the large- and short- single copy regions. The annotation included 136 genes, 90 protein-coding genes, 38 transfer, and 8 ribosomal RNAs and the sequence generated a distinct phyletic group within Asteraceae with the well-separated C. endivia and intybus species. SSR variants within the reference genome were mostly of tri-nucleotide type, and the cytosine to uracil (C/U) RNA editing recurred. The cp genome was nearly fully transcribed, hence sequence polymorphism was investigated by RNA-Seq of seven cultivar...

Source: Genes - September 21, 2023 Category: Genetics & Stem Cells Authors: Giulio Testone Michele Lamprillo Maria Gonnella Giuseppe Arnesi Anatoly Petrovich Sobolev Riccardo Aiese Cigliano Donato Giannino Tags: Article Source Type: research