Two-dimensional chromatography for enantiomeric analysis of mitotane and its metabolite o,p'-DDA in patients with adrenocortical carcinoma indicates enantioselective metabolism

In this study, we analyzed the enantiomers of mitotane and o,p'-DDA in the plasma of patients by a newly developed chiral-phase method employed in two-dimensional chromatography. Important differences were observed in the ratio of (S)/(R)-mitotane, which varied substantially from 1:1.2 to 1:10 whereas the (S)/(R)-o,p'-DDA ratio was relatively conserved, at approximately 2:1. These findings provide evidence for the enantioselective metabolism and provide a method for further analyses of mitotane and metabolites, which can explain the variation in the therapeutic response.PMID:37713949 | DOI:10.1016/j.bioorg.2023.106835

Source: Bioorganic Chemistry - September 15, 2023 Category: Chemistry Authors: Gabriela Stadler Alan de Almeida Veiga Claudia Rita Corso Camila Bach de Assis Beatriz de Toledo Nogueira Lucia Regina Rocha Martins Beatriz Cruz Bonk Fl ávia Lada Degaut Pontes Bonald Cavalcante de Figueiredo Lauro Mera de Souza Source Type: research