Label ‐Free and Colorimetric Detection of Influenza A Virus via Receptor‐Mediated Viral Fusion with Plasmonic Vesicles

This work presents a plasmonic vesicle-mediated fusogenic immunoassay (PVFIA) for the label-free and colorimetric detection of influenza A virus (IAV). PVFIA demonstrates excellent specificity in capturing the target, while the fusion assay with gold nanoparticle (GNP) encapsulating fusogenic plasmonic vesicles (PVs) enables visual detection. The results show a low detection limit (100.7919 EID50 mL−1) and good reliability (0.9901), indicating sensitivity 104.208 times higher than conventional immunoassay. AbstractThe rapid transmission and numerous re-emerging human influenza virus variants that spread via the respiratory system have led to severe global damage, emphasizing the need for detection tools that can recognize active and intact virions with infectivity. Here, this work presents a plasmonic vesicle-mediated fusogenic immunoassay (PVFIA) comprising gold nanoparticle (GNP) encapsulating fusogenic polymeric vesicles (plasmonic vesicles; PVs) for the label-free and colorimetric detection of influenza A virus (IAV). The PVFIA combines two sequential assays: a biochip-based immunoassay for target-specific capture and a PV-induced fusion assay for color change upon the IAV –PV fusion complex formation. The PVFIA demonstrates excellent specificity in capturing the target IAV, while the fusion conditions and GNP induce a significant color change, enabling visual detection. The integration of two consecutive assays results in a low detection limit (100.7919 EID50 mL−1)...
Source: Small - Category: Nanotechnology Authors: Tags: Research Article Source Type: research