GSE235229 A truncated HIV Tat demonstrates potent and specific latency reversal activity

Contributors : Filmon Eyassu ; Ellen Van Gulck ; Marion Pardons ; Erik Nijs ; Nick verheyen ; Koen Dockx ; Christel Van den Eynde ; Jerel Vega ; Eric Florence ; Brigitte Autran ; Nancy Archin ; David Margolis ; Kristine Katlama ; Chiraz Hamimi ; Ilse Van den Wyngaert ; Filmon Eyassu ; Linos Van de Kerckhove ; Daniel BodenSeries Type : Expression profiling by arrayOrganism : Homo sapiensThe barrier to HIV-1 functional cure is caused by a small pool of latently infected resting CD4 T-cells that persist under antiretroviral therapy. Notably this latent reservoir of infected cells will produce replication-competent infectious virus once prolonged suppressive HAART is withdrawn. The reactivation of HIV-1 gene expression in T-cells harboring latent provirus in HIV-1 patients under HAART will likely result in depletion of this latent reservoir due to cytopathic effects and immune clearance. Many studies have investigated small molecules that reactivate HIV-1 gene expression but to date no latency reversal agent (LRA) has been identified to be specific, non-toxic, and effective in primary T-cells isolated from HIV-1 infected individuals undergoing long-term HAART. Stochastic fluctuations in HIV-1 tat gene expression have been attributed to be essential in the viral progression to latency.We hypothesized that exposing Tat to latently infected CD4 T-cells will result in potent latency reversal. Our results indicate the capacity of an engineered Tat to reactivate HIV-1...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research