The impact of aerobic exercise training and crocin consumption on MuRf-1 level in skeletal muscle in high-fat fed ovariectomized rat models

AbstractEstrogen deficiency intensifies sarcopenia by activation of inflammatory and oxidative pathways. Aerobic exercise training and antioxidant/inflammatory supplements might reverse such outcomes or decline the degenerative pathway. Thus, we investigated the impact of aerobic training and crocin (CRO) consumption on skeletal muscle MuRf-1 and plasma antioxidant and inflammatory statues in ovariectomized (OVX) rat models fed on a high-fat diet (HFD). 72 female Wistar rats (200  ± 20 gr) were randomly divided into 9 groups: (1) non-OVX + standard chow (nOVX + SC), (2) nOVX + HFD, (3) high-intensity continuous training (HICT) + OVX + HFD + CRO, (4) HICT + OVX + HFD, (5) moderate-intensity continuous training (MICT) + OVX + HFD  + CRO, (6) MICT + OVX + HFD, (7) OVX + HFD + CRO, (8) OVX + SC, and (9) OVX + HFD. The OVX rats underwent surgery for menopause model induction. Immediately after the ovariectomy, rats were fed either by HFD or SC. Seven weeks later, exercise training (HICT/MICT; 8-week, 5- session, 12–30 m/min or 12–21 m/min) and CRO consumption (60 mg/kg) intervention per sessions were performed. The plasma levels of SOD, GSH, and TNF-α and muscle levels of MuRf-1 were assessed using the ELISA method. A two-tailedt-test and one-way ANOVA were applied for data analysis. There was not a significant difference in the protein levels of MuRF1 as the CRO caused an increase in the TNF- α leve...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research