Isolation, characterization, and evaluation of putative new bacteriophages for controlling bacterial spot on tomato in Brazil

AbstractBacterial spot is a highly damaging tomato disease caused by members of several species of the genusXanthomonas. Bacteriophages have been studied for their potential use in the biological control of bacterial diseases. In the current study, bacteriophages were obtained from soil and tomato leaves in commercial fields in Brazil with the aim of obtaining biological control agents against bacterial spot. Phage isolation was carried out by co-cultivation with isolates ofXanthomonas euvesicatoria pv.perforans, which was prevalent in the collection areas. In a host range evaluation, none of the phage isolates was able to induce a lytic cycle in all of the bacterial isolates tested. Inin vivo tests, treatment of susceptible bacterial isolates with the corresponding phage prior to application to tomato plants led to a reduction in the severity of the resulting disease. The level of disease control provided by phage application was equal to or greater than that achieved using copper hydroxide. Electron microscopy analysis showed that all of the phages had similar morphology, with head and tail structures similar to those of viruses belonging to the classCaudoviricetes. The presence of short, non-contractile tubular tails strongly suggested that these phages belong to the familyAutographiviridae. This was confirmed by phylogenetic analysis, which further revealed that they all belong to the genusPradovirus. The phages described here are closely related to each other and potenti...
Source: Archives of Virology - Category: Virology Source Type: research