Crosstalk between cholesterol and PIP < sub > 2 < /sub > in the regulation of Kv7.2/Kv7.3 channels

Biol Chem. 2023 Aug 8. doi: 10.1515/hsz-2023-0204. Online ahead of print.ABSTRACTThe activity of neuronal Kv7.2/Kv7.3 channels is critically dependent on PIP2 and finely modulated by cholesterol. Here, we report the crosstalk between cholesterol and PIP2 in the regulation of Kv7.2/Kv7.3 channels. Our results show that currents passing through Kv7.2/Kv7.3 channels in cholesterol-depleted cells, by acute application of methyl-β-cyclodextrin (MβCD), were less sensitive to PIP2 dephosphorylation strategies than those of control cells, suggesting that cholesterol depletion enhances the Kv7.2/Kv7.3-PIP2 interaction. In contrast, the sensitivity of Kv7.2/Kv7.3 channels to acute membrane cholesterol depletion by MβCD was not altered in mutant channels with different apparent affinities for PIP2.PMID:37552610 | DOI:10.1515/hsz-2023-0204
Source: Biological Chemistry - Category: Chemistry Authors: Source Type: research