Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil
Infect Genet Evol. 2023 Aug 4:105487. doi: 10.1016/j.meegid.2023.105487. Online ahead of print.ABSTRACTDENV-2 was the main responsible for a 70% increase in dengue incidence in Brazil during 2019. That year, our metagenomic study by Illumina NextSeq on serum samples from acute febrile patients (n = 92) with suspected arbovirus infection, sampled in 22 cities of the state of Mato Grosso (MT), in the middle west of Brazil, revealed eight complete genomes and two near-complete sequences of DENV-2 genotype III, one Human parvovirus B19 genotype I (5,391 nt) and one Coxsackievirus A6 lineage D (4,514 nt). These sequences share the aminoacidic identities of BR4 lineage on E protein domains I, II and III, and were included in a clade with sequences of the same lineage circulating in the southeast of Brazil in the same year. Nevertheless, 11/34 non-synonymous mutations are unique to three strains inthis study, distributed in the E (n = 6), NS3 (n = 2) and NS5 (n = 3) proteins. Other 14 aa changes on C (n = 1), E (n = 3), NS1 (n = 2), NS2A (n = 1) and NS5 (n = 7) were first reported in a genotype III lineage, having been already reported only in other DENV-2 genotypes. All 10 sequences have mutations in the NS5 protein (14 different aa changes), when compared with the respective reference sequences. Nine E protein aa changes, six of which are unique, found in two sequences, are in the ectodomain; where the E:M272T change is on the hinge of the E protein at domain II, in a region criti...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Marcelo Adriano Mendes Dos Santos Janeth Aracely Ramirez Pavon Lucas Silva Dias Ana Elisa Viniski Claudio Luis Campos Souza Elaine Cristina de Oliveira Verg ínia Correa de Azevedo Sandro Patroca da Silva Ana Cec ília Ribeiro Cruz Daniele Barbosa de Alme Source Type: research
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