Viruses, Vol. 15, Pages 1602: Porcine Circovirus 2 Increases the Frequency of Transforming Growth Factor- & beta; via the C35, S36 and V39 Amino Acids of the ORF4

Viruses, Vol. 15, Pages 1602: Porcine Circovirus 2 Increases the Frequency of Transforming Growth Factor-β via the C35, S36 and V39 Amino Acids of the ORF4
Viruses doi: 10.3390/v15071602
Authors:
Cheng Han
Weicheng Xu
Jianfang Wang
Xiaolin Hou
Shuanghai Zhou
Qinye Song
Xuewei Liu
Huanrong Li

Porcine circovirus 2 (PCV2) is one of the most important endemic swine pathogens, inducing immunosuppression in pigs and predisposing them to secondary bacterial or viral infections. Our previous studies show that PCV2 infection stimulated pig intestinal epithelial cells (IPEC-J2) to produce the secretory transforming growth factor-β (TGF-β), which, in turn, caused CD4+ T cells to differentiate into regulatory T cells (Tregs). This may be one of the key mechanisms by which PCV2 induces immunosuppression. Here, we attempt to identify the viral proteins that affect the TGF-β secretion, as well as the key amino acids that are primarily responsible for this occurrence. The three amino acids C35, S36 and V39 of the ORF4 protein are the key sites at which PCV2 induces a large amount of TGF-β production in IPEC-J2 and influences the frequency of Tregs. This may elucidate the regulatory effect of PCV2 on the Tregs differentiation from the perspective of virus structure and intestinal epithelial cell interaction, laying a theoretical foundation for improving the molecular mechanism of PCV2-induced intestinal mucosal...

Source: Viruses - July 22, 2023 Category: Virology Authors: Cheng Han Weicheng Xu Jianfang Wang Xiaolin Hou Shuanghai Zhou Qinye Song Xuewei Liu Huanrong Li Tags: Article Source Type: research