Critical appraisal tools used in systematic reviews of in vitro cell culture studies: A methodological study

AbstractSystematic reviews (SRs) of preclinical studies are marked with poor methodological quality. In vitro studies lack assessment tools to improve the quality of preclinical research. This methodological study aimed to identify, collect, and analyze SRs based on cell culture studies to highlight the current appraisal tools utilized to support the development of a validated critical appraisal tool for cell culture in vitro research. SRs, scoping reviews, and meta-analyses that included cell culture studies and used any type of critical appraisal tool were included. Electronic search, study selection, data collection and methodological quality (MQ) assessment tool were realized. Further, statistical analyses regarding possible associations and correlations between MQ and collected data were performed. After the screening process, 82 studies remained for subsequent analysis. A total of 32 different appraisal tools were identified. Approximately 60% of studies adopted pre-structured tools not designed for cell culture studies. The most frequent instruments were SYRCLE (n = 14), OHAT (n = 9), Cochrane Collaboration's tool (n = 7), GRADE (n = 6), CONSORT (n = 5), and ToxRTool (n = 5). The studies were divided into subgroups to perform statistical analyses. A significant association (OR = 5.00, 95% CI = 1.54–16.20,p = 0.008) was found between low MQ and chronic degenerative disorders as topic of SR. Several challenges in collecting informati...

Source: Research Synthesis Methods - July 19, 2023 Category: Chemistry Authors: Victor Paiva Barbosa, Bruna Bastos Silveira, Juliana Amorim dos Santos, Mylene Martins Monteiro, Ricardo D. Coletta, Graziela De Luca Canto, Cristine Miron Stefani, Eliete Neves Silva Guerra Tags: REVIEW Source Type: research