Targeted DNA N6 ‐methyladenine editing by dCas9 fused to METTL4 in the lepidopteran model insect Bombyx mori

We have established a novel CRISPR –dCas9–METTL4 epigenome editing tool that can methylate target regions to achieve site-specific DNA 6mA methylation in both hypermethylated and hypomethylated genes. Targeted methylation on genes by dCas9–METTL4 results in misexpression, allowing for the functional investigation of target gene s of interest in silkworm.

Source: Insect Science - July 18, 2023 Category: Biology Authors: Feng Chen, Hao Guo, Weiqun Lan, Mingyi Zhou, Wenjing Geng, Guanwang Shen, Ping Lin, Qingyou Xia, Ping Zhao, Zhiqing Li Tags: LETTER TO THE EDITOR Source Type: research