A novel transcription factor combination for direct reprogramming to a spontaneously contracting human cardiomyocyte-like state

The reprogramming of somatic cells to a spontaneously contracting cardiomyocyte-like state using defined transcription factors has proven successful in mouse fibroblasts. However, this process has been less successful in human cells, thus limiting the potential clinical applicability of this technology in regenerative medicine. We hypothesized that this issue is due to a lack of cross-species concordance between the required transcription factor combinations for mouse and human cells. To address this issue, we identified novel transcription factor candidates to induce cell conversion between human fibroblasts and cardiomyocytes, using the network-based algorithm Mogrify.

Source: Journal of Molecular and Cellular Cardiology - July 6, 2023 Category: Cytology Authors: Marisol Romero-Tejeda, Hananeh Fonoudi, Carly J. Weddle, Jean-Marc DeKeyser, Brian Lenny, K. Ashley Fetterman, Tarek Magdy, Yadav Sapkota, Conrad Epting, Paul W. Burridge Source Type: research