In vitro and in silico assessment of anti-quorum sensing activity of Naproxen against Pseudomonas aeruginosa

This study aimed to investigate the effect of naproxen on biofilm formation and QS-related virulence traits ofP. aeruginosa. Furthermore, the anti-QS potential of naproxen was evaluated using real-time PCR and molecular docking analysis. Our findings supported the anti-QS activity of naproxen, as evidenced by down-regulation of thelasI andrhlI genes expression as well as the attenuation of bacterial protease, hemolysin, pyocyanin, biofilm, and motility. Additionally, the high binding affinity of naproxen with QS regulatory proteins was determined in the molecular docking simulation. Altogether, these findings suggest that naproxen has a promising potential in inhibiting QS-associated traits ofP. aeruginosa.
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research