Physicochemical properties and Pb < sup > 2+ < /sup > adsorption capacity of freeze-dried hawthorn pectin fractions by gradient ethanol precipitation

Int J Biol Macromol. 2023 Jun 27:125581. doi: 10.1016/j.ijbiomac.2023.125581. Online ahead of print.ABSTRACTThree fractions of FHP20, FHP40 and FHP60 were obtained from freeze-dried hawthorn pectin by gradient ethanol precipitation (20-60 %), and their physicochemical properties and adsorption performance on Pb2+ were investigated. It was found that the content of galacturonic acid (GalA) and esterification of FHP fractions gradually reduced with the increase of ethanol concentration. FHP60 had the lowest molecular weight (60.69 × 103 Da), and the composition and proportion of monosaccharides were significantly different. The experimental results of Pb2+ adsorption showed that the adsorption process fitted well with the Langmuir monolayer adsorption and the pseudo-second-order models. Our findings suggested that pectin fractions with good homogeneity of molecular weight and chemical construction can be obtained by gradient ethanol precipitation, and hawthorn pectin could be developed as a potential adsorbent for Pb2+ removal.PMID:37385315 | DOI:10.1016/j.ijbiomac.2023.125581
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Source Type: research