< i > In vitro < /i > knockdown of < i > Ts < /i > DNase II-7 suppresses < i > Trichinella spiralis < /i > invasion into the host ’s intestinal epithelial cells

This study aimed to use RNA interference to verify our speculation thatTsDNase II-7 in 3-day old adultT.spiralis (Ad3) plays a role in intestinal invasion.TsDNase II-7-specific small interfering RNAs (siRNAs) were delivered into muscle larvae (MLs) to knockdownTsDNase II-7 expression by electroporation. Twenty-four hours later, the MLs transfected with 2 μM siRNA-841 exhibited decreased inTsDNase II-7 transcription and expression as compared to the control MLs. The knockdown ofTsDNase II-7 expression did not affect ML viability, and the low expression ofTsDNase II-7 still maintained in Ad3 recovered fromTsDNase II-7-RNAi-ML infected mice, resulting in a weakened ability of Ad3 to invade intestinal epithelial cells (IECs). These results indicated that knockdown ofTsDNase II-7 gene expression via RNA interference (RNAi) suppressed adult worm invasion and confirmed thatTsDNase II-7 plays a crucial role during the intestinal phase ofT.spiralis infections, which provided new candidate for vaccine development ofT.spiralis.

Source: PLoS Neglected Tropical Diseases - June 8, 2023 Category: Tropical Medicine Authors: Jing Wang Source Type: research