Antiproliferative activity of secondary metabolites isolated from Humboldtia unijuga Bedd.

AbstractHumboldtia unijuga Bedd. (Fabaceae), a tree species endemic to southern region of Western Ghats in India, is least explored for its chemical constituents and biological activities. Phytochemical analysis ofH. unijuga roots and stems resulted in the isolation of twenty-nine secondary metabolites with four new molecules,viz., methyl 3-(tetradecanoyloxy)olean-12-en-28-oate (3), lup-20(29)-en-3-yl 3-hydroxyheptadecanoate (26), methyl 3-(dodecanoyloxy)olean-12-en-28-oate (27), 28-oxoolean-12-en-3-yl myristate (28). Antiproliferative (cytotoxicity) activity of the twenty-nine isolated compounds was evaluated by MTT assay in five cancer cell lines,viz., C6, Hepa 1-6, RIN-5F, DLA and PC-12. These in vitro screening assays revealed promising anticancer activity of oleanolic acid-3-acetate (5) and (2S)-1-O-palmitoyl-2-O-(9Z-octadecenoyl)-3-O-(6 ’-sulfo-α-D-quinovopyranosyl) glycerol (16). The mechanism of anticancer activity of these two molecules was elucidated by phase contrast and fluorescent microscopy, caspase 3 activity assay and flow cytometric cell cycle/apoptotic analyses. These compounds demonstrated anticancer activity on C6 and DLA cells by inducing cell specific apoptosis. Briefly, this study led to the isolation of twenty nine secondary metabolites, with four new ones, fromH. unijuga, and two of the isolated molecules demonstrated promising anticancer activity.Graphical abstract

Source: Medicinal Chemistry Research - June 6, 2023 Category: Chemistry Source Type: research