The human papillomavirus18 E7 protein inhibits CENP-C binding to α-satellite DNA.

In this study, we first found that CENP-C could bind centromere α-satellite DNAs using ChIP analysis and HA-tagged CENP-C/nuc transfected 293T cells. We then investigated if HA-CENP-C/nuc binding to α-satellite DNAs was affected by the E7 proteins of high- or low-risk HPVs. We found that transfection of the FLAG tagged HPV 18 E7 inhibited the binding of HA-CENP-C/nuc to α-satellite DNAs. This finding was confirmed in HeLa S3 cells transfected with siRNA targeted to HPV18 E7 expression. We therefore speculate that altered function of kinetochores as a result of inhibition of CENP-C and α-satellite DNA binding may be associated with the chromosomal abnormalities observed in HPV18-positive cancers. PMID: 25997930 [PubMed - as supplied by publisher]
Source: Virus Research - Category: Virology Authors: Tags: Virus Res Source Type: research