Mycotoxigenic fungal growth inhibition and multi-mycotoxin reduction of potential biological control agents indigenous to grain maize

AbstractThe present work investigated the potential of fungal species from grain maize farms in Malaysia as antagonists against the indigenous mycotoxigenic fungal species and their subsequent mycotoxin production. Dual-culture assay was conducted on grain maize agar (GMA) with 12 strains of potential fungal antagonists namelyBjerkandra adusta,Penicillium janthinellum,Schizophyllum commune,Trametes cubensis,Trichoderma asperelloides,Trichoderma asperellum,Trichoderma harzianum, andTrichoderma yunnanense against seven mycotoxigenic strains namelyAspergillus flavus,Aspergillus niger,Fusarium verticillioides, andFusarium proliferatum producing aflatoxins, ochratoxin A, and fumonisins, respectively. Based on fungal growth inhibition,Trichoderma spp. showed the highest inhibitory activity (73 –100% PIRG, Percentage Inhibition of Radial Growth; 28/0 ID, Index of Dominance) against the tested mycotoxigenic strains. Besides,B. adusta andTra. cubensis showed inhibitory activity against some of the tested mycotoxigenic strains. All fungal antagonists showed varying degrees of mycotoxin reduction. Aflatoxin B1 produced byA. flavus was mainly reduced byP. janthinellum,Tra. cubensis, andB. adusta to 0  ng/g. Ochratoxin A produced byA. niger was mainly reduced byTri. harzianum andTri. asperellum to 0  ng/g. Fumonisin B1 and FB2 produced byF. verticillioides was mainly reduced byTri. harzianum,Tri. asperelloides, andTri. asperellum to 59.4 and 0  µg/g, respectively. Fumonisin B1 and F...
Source: Mycotoxin Research - Category: Toxicology Source Type: research