Metabolic changes during larval –pupal metamorphosis of Helicoverpa armigera

Helicoverpa armigera larvae quickly grow and accumulate carbohydrates and lipids with rapid aerobic glycolysis during the feeding stage. Aerobic glycolysis is rapidly inactivated, and lipid degradation is rapidly activated, during the larval nonfeeding stage. 20-hydroxyecdysone cooperates with carnitine to promote lipid utilization. AbstractEnergy metabolism is essential for insect metamorphosis. The accumulation and utilization of energy is still not completely clear during larval –pupal metamorphosis of holometabolous insects. We used metabolome and transcriptome analysis to reveal key metabolic changes in the fat body and plasma and the underlying metabolic regulation mechanism ofHelicoverpa armigera, an important global agricultural insect pest, during larval –pupal metamorphosis. During the feeding stage, activation of aerobic glycolysis provided intermediate metabolites and energy for cell proliferation and lipid synthesis. During the non-feeding stages (the initiation of the wandering stage and the prepupal stage), aerobic glycolysis was suppressed, while, triglyceride degradation was activated in the fat body. The blocking of metabolic pathways in the fat body was probably caused by 20-hydroxyecdysone-induced cell apoptosis. 20-hydroxyecdysone cooperated with carnitine to promote the degradation of triglycerides and the accumulation of acylca rnitines in the hemolymph, allowing rapid transportation and supply of lipids from the fat body to other organs, which prov...

Source: Insect Science - May 18, 2023 Category: Biology Authors: Xinxin Gao, Jihong Zhang, Qilian Qin, Peipei Wu, Huan Zhang, Qian Meng Tags: ORIGINAL ARTICLE Source Type: research