Haematologic profile and histomorphologic assessment of the pancreas and liver of alloxan-induced diabetic rats treated with graded concentration of quail egg

This study was designed to investigate the effect of quail egg on the haematological parameters and the histopathology changes of the pancreas and liver of alloxan-induced diabetic Wistar albino male rats. Thirty albino rats with a mean weight of 160 ± 15 g were randomly selected into five groups, each with six rats. Groups 2, 3, 4 and 5 were induced with diabetes by intraperitoneal injection of alloxan at a dose of 160 mg/kg. Upon confirmation of diabetes (fasting blood sugar (FBS) >126 mg/dl), rats in groups 2, 3 and 4 were orally administered with 30, 15 and 7.5 mg/ml of quail egg. Group 1 and 5 rats were administered with distilled water to serve as positive and negative controls, respectively. The treatment lasted for 21 days, and blood samples for haematology [red blood cell (RBC), white blood cell (WBC), haemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV) and mean corpuscular haemoglobin concentration (MCHC)] analyses were collected on days 7, 14 and 21. On the 21st day, two rats per group were humanely sacrificed and the vital organs (pancreas and liver) were collected for histopathology. Haematologic results obtained showed marked reduction (p < 0.05) in RBC count, PCV, Hb and MCHC, a significant increase (p < 0.05) in MCV and no significant difference (p > 0.05) in the WBC of the induced animals when compared with the positive control. However, on administration of the quail egg at 30 mg/ml, there was a signif...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research