Transfection efficacy and drug release depends upon the PEG derivative in cationic lipoplexes: Evaluation in 3D in vitro model and in vivo

AbstractThe goal of the study was to estimate transfection efficacy and drug release in function of the PEG derivative in cationic liposomes and lipoplexes in both 2D and 3D in vitro models as well as in a mouse model (in vivo). For this purpose, cationic PEGylated nanocarriers based on OrnOrnGlu(C16H33)2 lipopeptides were fabricated and characterized. The nanocarriers were loaded with DNA plasmid pGL3 or with siRNA targeting 5 ′-UTR region of Hepatitis C virus, and their transfection efficacies were studied by luciferase test or by PCR technique, respectively. The pGL3-lipoplexes containing PEG derivativeb (6  mol % PEG) were selected as the most promising nanocarriers for further in vivo study. In vitro cytotoxicity assay of the pGL3-lipoplexes with the PEG derivativeb showed 2- and 1.5-fold enhancements of IC50 levels for HEK293T and HepG2 cells, respectively. Accumulation of the liposomes in the cells was studied by confocal microscopy using both 2D (monolayer culture) and 3D (multicellular spheroids) in vitro models. The PEGylated liposomes were found to penetrate cells more slowly than unmodified ones (without PEG). Thus, maximum liposomes in the HEK293T cells was observed after 1 and 3  h in the case of 2D and 3D in vitro models, respectively. Biodistribution study in mice showed that the PEGylated lipoplexes containing the PEG derivativeb were eliminated from the bloodstream more slowly, namely with the doubled half-life time, than unmodified ones. Thus, the enh...

Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - May 4, 2023 Category: Materials Science Authors: Gileva A. M., Koloskova O. O., Nosova A. S., Vishniakova L. I., Simonova V. A., Kurbanova L. A., Egorenkov E. A., Smirnov V. V., Budanova U. A., Sebyakin Yu L., Suzina N. E., Khaitov M. R., Markvicheva E. Tags: RESEARCH ARTICLE Source Type: research