Mir-629 Repressed LATS2 Expression and Promoted the Proliferation of Prostate Cancer Cells
Horm Metab Res DOI: 10.1055/a-2065-0954MicroRNAs (miRNAs) have been discovered to play critical role in regulating
prostate cancer (PC) progression. The function role of miR-629 in tumor
progression of PC has not been studied. Here, we found that miR-629 was markedly
upregulated in PC as determined using the cancer genome atlas (TCGA) dataset,
clinical tissues, and cell lines. Functional analysis (MTT assays, colony
formation assays, soft agar growth assay and BrdU incorporation assay) indicated
that overexpression of miR-629 was drastically promoted, while miR-629-in
significantly suppressed cell proliferation. LATS2 was predicted as a direct
target of miR-629 and was confirmed by western blot and dual luciferase assay.
Through downregulation of large tumor suppressor 2 (LATS2) by overexpression of
miR-629, the p21 mRNA and protein were decreased while the Cyclin D3 mRNA and
protein were enhanced, suggesting promoting of cell proliferation process.
Additionally, knockdown of LATS2 reversed the inhibitory effect by miR-629-in in
PC. Our study indicated that miR-629 might serve as a new promising target for
PC treatment. [...] Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, GermanyArticle in Thieme eJournals: Table of contents | Abstract | Full text
Source: Hormone and Metabolic Research - Category: Endocrinology Authors: Li, Yuqiao Zeng, Sha Cao, Longbin Tags: Original Article: Endocrine Research Source Type: research