CCDC3 Gene Regulates the Proliferation of Breast Cancer Cells

We studied the effect ofCCDC3 on the viability of human breast cancer cell line MDA-MB-231. The levels ofCCDC3 mRNA and the corresponding protein in MDA-MB-231, MCF-7, T-47D, and HCC1937 cell lines were measured by reverse transcription quantitative real-time PCR and Western blotting. Since MDA-MB-231 cells had higher expression of mRNACCDC3 andCCDC3 protein, we used this cell line for transfection with small interfering RNA by lentivirus. Cell Counting Kit-8 and clone formation assay were used to detect the effects ofCCDC3 knockdown on cell viability; flow cytometry was used to detect the effects ofCCDC3 knockdown on cell apoptosis and cell cycle. In MDA-MB-231 cell line, the CCDC3 protein level was significantly down-regulated afterCCDC3 knockdown in comparison with the control group (p<0.05). The cell viability and the number of clones in theCCDC3 knockdown group were significantly reduced (p<0.05), while the apoptosis rate significantly increased (p<0.05). Thus, afterCCDC3 knockdown, cell viability is weakened in MDA-MB-231 cells, and cell apoptosis rate is increased. Therefore,CCDC3 gene is promising as a new candidate target for BC treatment.
Source: Bulletin of Experimental Biology and Medicine - Category: Biology Source Type: research