Rapamycin does not act as a dietary restriction mimetic in the protection against ischemia reperfusion injury

This study examines the effect of rapamycin on renal IRI. Methods Mice were divided in four groups : Ad libitum(AL), Fasted (F), AL treated with rapamycin (AL+R) and F treated with rapamycin (F+R). Rapamycin was administered intraperitoneally 24h before bilateral renal IRI was induced. Survival was monitored for 7 days. Renal cell death, regeneration, an d mTOR activity were determined 48h after reperfusion. Oxidative stress resistance of HK-2 and PTEC cells after rapamycin treatment was determined. Results All F and F+R mice survived the experiment. Although rapamycin substantially downregulated mTOR-activity, survival in the AL+R group was simi lar to AL (10%). Renal regeneration was significantly reduced in AL+R but not in F+R. After IRI (48h), pS6K/S6K ratio was lower in F, F+R and AL+R groups compared to AL fed animals (p=0.02). In vitro, rapamycin also significantly downregulated mTOR-activity (p
Source: European Surgical Research - Category: Surgery Source Type: research