Multi-Function of a New Bioactive Secondary Metabolite Derived from Endophytic Fungus < em > Colletotrichum acutatum < /em > of < em > Angelica sinensis < /em >

J Microbiol Biotechnol. 2023 Jan 6;33(6):1-17. doi: 10.4014/jmb.2206.06010. Online ahead of print.ABSTRACTIn the current study we assessed a new crystallized compound, 5-(1-hydroxybutyl)-4-methoxy-3- methyl-2H-pyran-2-one (C-HMMP), from the endophytic fungus Colletotrichum acutatum residing in the medicinal plant Angelica sinensis for its in vitro antimicrobial, antibiofilm, antioxidant, antimalarial, and anti-proliferative properties. The promising compound was identified as C-HMMP through antimicrobial-guided fraction. The structure of C-HMMP was unambiguously confirmed by 2D NMR and HIRS spectroscopic analysis. Antimicrobial property testing of C-HMMP showed it to be effective against a variety of pathogenic bacteria and fungi with MICs ranging from 3.9 to 31.25 μg/ml. The compound displayed excellent antibiofilm activity against C. albicans, S. aureus, and K. pneumonia. Furthermore, the antimalarial and radical scavenging activities of C-HMMP were clearly dose-dependent, with IC50 values of 0.15 and 131.2 µg/ml, respectively. The anti-proliferative activity of C-HMMP against the HepG-2, HeLa, and MCF-7 cell lines in vitro was investigated by MTT assay, revealing notable anti-proliferative activity with IC50 values of 114.1, 90, and 133.6 μg/ml, respectively. Moreover, C-HMMP successfully targets topoisomerase I and demonstrated beneficial anti-mutagenicity in the Ames test against the reactive carcinogenic mutagen, 2-aminofluorene (2- AF). Finally, the compound inhibit...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Source Type: research