Viruses, Vol. 15, Pages 617: Chinese Giant Salamander Iridovirus 025L Is a Viral Essential Gene

Viruses, Vol. 15, Pages 617: Chinese Giant Salamander Iridovirus 025L Is a Viral Essential Gene Viruses doi: 10.3390/v15030617 Authors: Zijing Liu Daofa Xie Shirong Nong Yingzi Wu Suxian Huang Xianhui He Tianhong Zhou Wei Li Ranavirus is a large nucleocytoplasmic DNA virus. Chinese giant salamander iridovirus (CGSIV) belongs to the ranavirus genus, and its replication involves a series of essential viral genes. Viral PCNA is a gene closely associated with viral replication. CGSIV−025L also encodes PCNA−like genes. We have described the function of CGSIV−025L in virus replication. The promoter of CGSIV−025L is activated during viral infection, and it is an early (E) gene that can be effectively transcribed after viral infection. CGSIV−025L overexpression promoted viral replication and viral DNA replication. siRNA interfered with CGSIV−025L expression and attenuated viral replication and viral DNA replication. The Δ025L−CGSIV strain with the deletion of CGSIV−025L could not replicate normally and could be rescued by the replenishment of 025L. CGSIV−025L was proven to be an essential gene for CGSIV by overexpression, interference, and deletion mutation experiments. CGSIV−025L was found to interact with CGSIV−062L by yeast two−hybrid, CoIP, and GST pulldown. Thus, the current stu...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research