Molecularly imprinted miniature electrochemical biosensor for SARS-CoV-2 spike protein based on Au nanoparticles and reduced graphene oxide modified acupuncture needle

Bioelectrochemistry. 2023 Jan 27;151:108375. doi: 10.1016/j.bioelechem.2023.108375. Online ahead of print.ABSTRACTAccurate detection of SARS-CoV-2 spike (SARS-CoV-2-S) protein is of clinical significance for early diagnosis and timely treatment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Herein, a surface molecularly imprinted miniature biosensor was fabricated. Au nanoparticles (AuNPs), reduced graphene oxide (rGO), poly(methylene blue)/poly(ionic liquids) and poly(ionic liquids) were successively electrodeposited onto the pinpoint of an acupuncture needle (AN). The molecularly imprinted miniature biosensor was obtained after the template of SARS-CoV-2-S protein was removed, which could be used for sensitive detection of SARS-CoV-2-S protein. The linear range and limit of detection (LOD) were 0.1 ∼ 1000 ng mL-1 and 38 pg mL-1, respectively, which were superior to other molecularly imprinted biosensors previously reported. The developed miniature biosensor also exhibited high specificity and stability. The reliability of the biosensor was evaluated by the detection of SARS-CoV-2-S protein in clinical serum samples.PMID:36716516 | PMC:PMC9883973 | DOI:10.1016/j.bioelechem.2023.108375

Source: Bioelectrochemistry - January 30, 2023 Category: Biochemistry Authors: Xu Yang Zheng-Zhi Yin Guojun Zheng Min Zhou Hongyu Zhang Junyao Li Wenrong Cai Yong Kong Source Type: research