An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation

Conclusions Purification of untagged recombinant PHGDH eliminates the need to use an epitope tag for enzyme studies. Furthermore, while tagged PHGDH retains some ability to convert 3PG to PHP, the structural alterations caused by including an epitope tag disrupts the ability of PHGDH to sustain cancer cell proliferation.
Source: Cancer and Metabolism - Category: Cancer & Oncology Source Type: research