Molecules, Vol. 27, Pages 8101: In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient

Molecules, Vol. 27, Pages 8101: In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient Molecules doi: 10.3390/molecules27228101 Authors: José Quiles Maria Cabrera Jonathan Jones Menelaos Tsapekos Nuria Caturla The beauty industry is actively searching for solutions to prevent skin aging. Some of the crucial elements protecting cells from the aging process are telomere shortening, telomerase expression, cell senescence, and homeostasis of the redox system. Modification of these factors using natural antioxidants is an appealing way to support healthy skin aging. Therefore, in this study, we sought to investigate the antiaging efficacy of a specific combination of four botanical extracts (pomegranate, sweet orange, Cistanche and Centella asiatica) with proven antioxidant properties. To this end, normal human dermal fibroblasts were used as a cell model and the following studies were performed: cell proliferation was established by means of the MTT assay and the intracellular ROS levels in stress-induced premature senescence fibroblasts; telomere length measurement was performed under standard cell culture conditions using qPCR and under oxidative stress conditions using a variation of the Q-FISH technique; telomerase activity was examined by means of Q-TRAP; and AGE quantification was completed by means of ELISA assay in UV-irradiated fibroblasts. As a result, the botanical blend significantly reversed the H2O2-induced ...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research