Molecular detection of Helicobacter spp. and Fusobacterium gastrosuis in pigs and wild boars and its association with gastric histopathological alterations

AbstractBesidesHelicobacter pylori, a Gram-negative bacterium that may cause gastric disorders in humans, non-Helicobacter pylori helicobacters (NHPH) may also colonize the stomach of humans and animals. In pigs,H. suis can induce gastritis and may play a role in gastric ulcer disease, possibly in association withFusobacterium gastrosuis. In the present study, gastric samples from 71 slaughtered pigs and 14 hunted free range wild boars were tested for the presence of DNA ofF. gastrosuis and gastricHelicobacter species associated with pigs, dogs cats and humans, using species-specific PCR assays, followed by sequencing of the amplicon. These gastric samples were also histopathologically evaluated. Almost all the pigs presented gastritis (95.8%).Helicobacter spp. were detected in 78.9% andF. gastrosuis in 35.2% of the animals.H. suis was the most frequently identifiedHelicobacter species (57.7% of the animals), followed by aH. pylori-like species (50.7%) and less oftenH. salomonis andH. felis (each in 2.8% of the animals).H. suis was most often detected in the glandular (distal) part of the stomach (pars oesophagea 9.9%, oxyntic mucosa 35.2%, antral mucosa 40.8%), while theH. pylori-like species was mainly found in the non-glandular (proximal) part of the stomach (pars oesophagea 39.4%, oxyntic mucosa 14.1%, antral mucosa 4.2%). The great majority of wild boars were also affected with gastritis (71.4%) andHelicobacter spp. andF. gastrosuis were detected in 64.3% and 42.9% of th...
Source: Veterinary Research - Category: Veterinary Research Source Type: research