Effects of Surface Prereacted Glass on Saliva-Derived Polymicrobial Biofilms in an Active Attachment Biofilm Model

Bioactive restorative materials are being developed to either influence the de/remineralization balance of the dental hard tissues locally or to release components that interact with the oral microbiota. Surface prereacted glass (S-PRG, Shofu, Japan) is a material that may influence both processes. S-PRG releases fluoride, which can interact with the de/remineralization process, and a range of other compounds that may influence the oral microbiota. In the current study, several experiments were performed to investigate the potential of S-PRG to influence both the growth and lactic acid production of saliva-derived polymicrobial biofilms. Biofilm formation was studied using the Amsterdam Active Attachment model. An eluate of the S-PRG particles was tested by adding it to the growth medium or by exposing the biofilms to it for 1 h. The effect of S-PRG particles was tested by adding the particles to the growth medium. The current experiments showed that the presence of S-PRG eluate in the medium influenced biofilm growth and lactic acid production even at low concentrations. The composition of the biofilms changed in the presence of S-PRG eluate, even at concentrations of S-PRG eluate at which biofilm viability was not affected. Treatment of developing biofilms with S-PRG eluate did neither show an effect on biofilm viability nor on lactic acid production. The addition of S-PRG particles to the growth medium resulted in both a lower biofilm viability and lower lactic acid produc...
Source: Caries Research - Category: Dentistry Source Type: research