Examination of pretreatment methods for DNA extraction from nails

In this study, we examined whether using the HOrizontal Nail MAshing (HONMA) method, in which pressure is applied to the nail to crush it flat and increase its surface area, would improve DNA extraction efficiency. Fingernails (5mg) provided by ten volunteers were used as samples. Nail pieces (1 –3 pieces), shredded with nail clippers, were thinly stretched by applying 2 t of pressure to each piece using a hydraulic press.

Source: Forensic Science International: Genetics Supplement Series - September 30, 2022 Category: Forensic Medicine Authors: Eriko Ochiai, Minoru Asogawa, Wataru Irie, Chizuko Sasaki, Naomi Nakamaru, Momoko Sakamoto, Junpei Nagato, Junichiro Saito, Masatoshi Sugisawa, Fumiko Satoh Source Type: research