The structure of tubulin-binding cofactor A from Leishmania major infers a mode of association during the early stages of microtubule assembly
This study provides a reagent and template to support further work in this area.
We examined the pattern of toll-like receptor 2 (TLR2), TLR4, TLR7, interleukin- (IL-) 10, interferon gamma (IFN-γ), and (program death ligand) PD-L1 gene expression in LST-positive reactions and paired normal-looking skin of nine infected Ibizan hound dogs. Healthy skin from ten seronegative dogs from a nonendemic area was analysed as a negative control. Immune gene expressions were examined by quantitative PCR (qPCR) analysis. LST-positive reactions presented significant upregulation of TLR2, TLR4, IL-10, IFN-γ, and PD-L1 and downregulation of TLR7 when compared with healthy skin of seronegative control dogs ...
Publication date: Available online 25 March 2020Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Carlos A.H. Fernandes, Edna Gicela O. Morea, Gabriel A. dos Santos, Vitor L. da Silva, Marina Roveri Vieira, Maria Alejandra Viviescas, Jean Chatain, Aurélie Vadel, Carole Saintomé, Marcos Roberto M. Fontes, Maria Isabel Nogueira Cano
Publication date: 24 March 2020Source: Cell Reports, Volume 30, Issue 12Author(s): Inês Mesquita, Carolina Ferreira, Diana Moreira, George Eduardo Gabriel Kluck, Ana Margarida Barbosa, Egídio Torrado, Ricardo Jorge Dinis-Oliveira, Luís Gafeira Gonçalves, Charles-Joly Beauparlant, Arnaud Droit, Luciana Berod, Tim Sparwasser, Neelam Bodhale, Bhaskar Saha, Fernando Rodrigues, Cristina Cunha, Agostinho Carvalho, António Gil Castro, Jérôme Estaquier, Ricardo Silvestre
Publication date: Available online 24 March 2020Source: Biological ControlAuthor(s): Tharaka Wijerathna, Samadhi Gunathunga, Nayana Gunathilaka
ConclusionsOur data indicates that dietary glutamine supplementation may act as a promising adjuvant for the treatment of visceral leishmaniasis.
ConclusionOur results support the role ofPh.longicuspis,Ph.perfiliewi, andPh.perniciosus inL.infantum transmission. Furthermore, these species could be involved inL.major andL.killicki life cycles. The combination of the parasite detection and the blood meal analysis in this study highlights the incrimination of the identified vertebrate inLeishmania transmission. In addition, we quantify for the first time the parasite load in naturally infected sand flies caught in Tunisia. These findings are relevant for a better understanding ofL.infantum transmission cycle in the country. Further investigations and control measures ar...
ConclusionsThese results demonstrate that the thymus of dogs can be parasitized byL. infantum, which may generate inflammatory reactions leading to alterations in thymic microarchitecture.
This study reveals greater genetic diversity than suggested by geographically-focused studies, provides a resource of genomic variation for future work and sets the scene for a new understanding of the evolution and genetics of theLeishmania donovani complex.
FC, Dos Santos ES Abstract Leishmaniosis is caused by the protozoa of the genus Leishmania with a wide spectrum of clinical and epidemiological manifestations which are characterized into four clinical groups: cutaneous, mucocutaneous, diffuse cutaneous, and visceral. American visceral leishmaniosis (AVL) or visceral leishmaniosis (VL) has been known as the most severe form of the disease. However, despite the growing number of people exposed to the infection risk and the great effort done by the scientific community worldwide to significantly increase the knowledge about these diseases, there is no vaccine capabl...
In conclusion, our findings indicate that these aptamers could be used for LiH3 histone detection and, in consequence, as potential biosensing molecules in a diagnostic tool for leishmaniasis. PMID: 32200890 [PubMed - in process]