Isolation of mitochondria-derived mitovesicles and subpopulations of microvesicles and exosomes from brain tissues

Nature Protocols, Published online: 12 August 2022; doi:10.1038/s41596-022-00719-1This protocol describes the isolation from brain tissue of extracellular vesicle subpopulations, including microvesicles, exosomes and mitochondria-derived mitovesicles, using a high-resolution (iodixanol) density step gradient. Extracellular vesicle characterization and analysis are also presented.

Source: Nature Protocols - August 12, 2022 Category: Biomedical Science Authors: Pasquale D ’Acunzo Yohan Kim Jonathan M. Ungania Roc ío Pérez-González Chris N. Goulbourne Efrat Levy Source Type: research