Molecules, Vol. 27, Pages 4403: Development and Validation of the UPLC-DAD Methodology for the Detection of Triterpenoids and Phytosterols in Fruit Samples of Vaccinium Macrocarpon Aiton and Vaccinium Oxycoccos L.

Molecules, Vol. 27, Pages 4403: Development and Validation of the UPLC-DAD Methodology for the Detection of Triterpenoids and Phytosterols in Fruit Samples of Vaccinium Macrocarpon Aiton and Vaccinium Oxycoccos L.
Molecules doi: 10.3390/molecules27144403
Authors:
Rima Sedbare
Lina Raudone
Vaidotas Zvikas
Jonas Viskelis
Mindaugas Liaudanskas
Valdimaras Janulis

Cranberries are used in the production of medicinal preparations and food supplements, which highlights the importance of triterpene compounds determination in cranberry fruit raw material. The aim of our study was to develop and validate for routine testing suitable UPLC-DAD methodology for the evaluation of triterpene acids, neutral triterpenoids, phytosterols, and squalene content in cranberry samples. The developed and optimized UPLC-DAD methodology was validated according to the guidelines of the International Council for Harmonization (ICH), evaluating the following parameters: range, specificity, linearity (R2 > 0.999), precision, LOD (0.27–1.86 µg/mL), LOQ (0.90–6.18 µg/mL), and recovery (80–110%). The developed and validated technique was used for the evaluation of triterpenic compounds in samples of Vaccinium macrocarpon and Vaccinium oxycoccos fruits, and their peels, pulp and seeds. The studied chromatogram profiles of Vaccinium macrocarpon and Vaccinium oxycoccos were identical but differed in the areas of the ...

Source: Molecules - July 9, 2022 Category: Chemistry Authors: Rima Sedbare Lina Raudone Vaidotas Zvikas Jonas Viskelis Mindaugas Liaudanskas Valdimaras Janulis Tags: Article Source Type: research