Comparative evaluation of two rapid Salmonella-IgM tests and blood culture in the diagnosis of enteric fever

Conclusion: Rapid Salmonella-IgM tests offer an advantage of increased sensitivity, rapidity, early diagnosis and simplicity over blood culture.
Source: Indian Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research

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Abstract Enteric fever is one of the leading causes of infection and subsequent fatality (greater than 1.8 million) (WHO 2018), especially in the developing countries due to contaminated water and food inter twinned with unhygienic practices. Clinical gold standard technique of culture-based method followed by biochemical tests demand 72+ hours for diagnosis while newly developed techniques (like PCR, RT-PCR, DNA microarray etc.) suffer from high limit of detection or involve high-cost infrastructure or both. In this work, a quick and highly specific method, SMOL was established for simultaneous detection of Salmo...
Source: Biomedical Microdevices - Category: Biomedical Engineering Authors: Tags: Biomed Microdevices Source Type: research
Vaccination against Salmonella Typhi using the Vi capsular polysaccharide, a T-cell independent antigen, can protect from the development of typhoid fever. This implies that antibodies to Vi alone can protect in the absence of a T cell-mediated immune response; however, protective Vi antibodies have not been well-characterized. We hypothesized that variability in the biophysical properties of vaccine-elicited antibodies, including subclass distribution and avidity, may impact protective outcomes. To interrogate the relationship between antibody properties and protection against typhoid fever, we analyzed humoral responses ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 22 October 2019Source: Microbial PathogenesisAuthor(s): Chandran Sivasankar, Nisha Kumari Jha, Ruchira Ghosh, Prathapkumar Halady ShettyAbstractSalmonella enterica Typhi and Paratyphi A are food borne pathogens causing typhoid, which is one of the most important food borne disease in the developing world. S. Typhi and S. Paratyphi A are of much concern as multi drug resistance has been on the rise. The current study is aimed to screen phytochemicals for anti quorum sensing (QS) activity against S. Typhi and S. Paratyphi A. Upon screening with swarming assay, tannic acid (TA) showed highes...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: Available online 15 October 2019Source: Veterinary MicrobiologyAuthor(s): João Bettencourt Cota, Vanessa Ferreira da Silva, Lélia Chambel, Maria Gabriela Veloso, Madalena Vieira-Pinto, Manuela OliveiraAbstractPork is one of the most common vehicles of non-typhoid foodborne Salmonella, with the slaughterhouse representing a key point for the infection of pigs and carcass contamination. By comparing matching samples taken from animals at the dirty (skin) and clean (inner and outer carcass surface) areas of the slaughterline, this study aimed to assess potential Salmonella contamination routes ...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
in MB Abstract It is widely accepted that CD4+ and CD8+ T-cells play a significant role in protection against Salmonella enterica serovar Typhi (S. Typhi), the causative agent of the typhoid fever. However, the antigen specificity of these T-cells remains largely unknown. Previously, we demonstrated the feasibility of using a recombinant Escherichia coli (E. coli) expression system to uncover the antigen specificity of CD4+ and CD8+ T cells. Here, we expanded these studies to include the evaluation of 12 additional S. Typhi proteins: 4 outer membrane proteins (OmpH, OmpL, OmpR, OmpX), 3 Vi-polysaccharide biosynthe...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
Source: Infection and Drug Resistance - Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
Abstract Salmonella typhi is a causative organism for typhoid fever. Free Vi capsular polysaccharide (Vi) is licensed for use as vaccine for typhoid fever in individuals 2 years of age and older, which has limited memory response. There is dire need of protein or peptide as conjugate partner with Vi polysaccharide to improve shortcomings of Vi vaccine. Prediction of immunogenic peptide was deduced by program T sites. Carbodiimide mediated conjugation of Vi polysaccharide with OmpCp was performed utilizing ADH as linker. Immune response of Vi-conjugates along with control group was tested in mice. Ig and IgG antibo...
Source: Biologicals : Journal of the International Association of Biological Standardization - Category: Biotechnology Authors: Tags: Biologicals Source Type: research
Publication date: Available online 30 September 2019Source: Journal of Integrative MedicineAuthor(s): Gaëtan Olivier Fankem, Michel Archange Fokam Tagne, Paul Aimé Noubissi, Angèle Foyet Fondjo, Idrice Kamtchouing, Adela Ngwewondo, Henri Wambe, Joseph Ngakou Mukam, René KamgangAbstractObjectiveInfectious diseases such as typhoid fever leads to the formation of free radicals which can damage the body. Many medicinal plants have antioxidant molecules that neutralize free radicals. The present work evaluated the antioxidant activity and histopathological effects of the dichloromethane fraction of Dic...
Source: Journal of Integrative Medicine - Category: Complementary Medicine Source Type: research
ConclusionThe current findings provide valuable data for understanding the multidrug resistance and pathogenic characteristics of clinical Salmonella Paratyphi A isolates.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
by Danielle J. Ingle, Satheesh Nair, Hassan Hartman, Philip M. Ashton, Zoe A. Dyson, Martin Day, Joanne Freedman, Marie A. Chattaway, Kathryn E. Holt, Timothy J. DallmanSalmonella enterica serovar Typhi (S. Typhi) is the causative agent of typhoid fever, a systemic human infection with a burden exceeding 20 million cases each year that occurs disproportionately among children in low and middle income countries. Antimicrobial therapy is the mainstay for treatment, but resistance to multiple agents is common. Here we report genotypes and antimicrobial resistance (AMR) determinants detected from routine whole-genome sequencin...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
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