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Engineering, expression and purification of a chimeric fibrin-specific streptokinase
Publication date: Available online 3 August 2016 Source:Protein Expression and Purification Author(s): Mohammad Naser Taheri, Abbas Behzad-Behbahani, Gholamreza Rafiei Dehbidi, Saeede Salehi, Sedigheh Sharifzadeh Streptokinase is a valuable fibrinolytic agent used to cope with myocardial infarction and brain stroke. Despite its high efficiency in dissolving blood clots, streptokinase (SK) has no specificity in binding fibrin, causing some problems such as internal bleedings following its administration. To make streptokinase fibrin specific and limit the fibrinolytic process to the clot location, we engineered a chimeric ...
Source: Protein Expression and Purification - August 2, 2016 Category: Biochemistry Source Type: research

Cloning, Prokaryotic Expression and Functional Analysis of Squalene Synthase (SQS) in Magnolia officinalis
In this study, a full-length cDNA of squalene synthase was cloned from M. officinalis and designated MoSQS (GenBank accession no.KT223496). The gene contains a 1240-bp open reading frame and it encodes a protein with 409 amino acids. Bioinformatic and phylogenetic analysis clearly suggested that MoSQS shared high similarity with squalene synthases among other plants. Prokaryotic expression showed that a transmembrane domain-deleted (385–409 aa) MoSQS mutant (MoSQSΔTM) could be expressed in its soluble form in Escherichia coli Transetta (DE3). GC-MS analysis showed that squalene was detected in an in vitro reaction mixtu...
Source: Protein Expression and Purification - December 13, 2015 Category: Biochemistry Source Type: research

An experimental system for controlled exposure of biological samples to electrostatic discharges.
Abstract Electrostatic discharges occur naturally as lightning strokes, and artificially in light sources and in materials processing. When an electrostatic discharge interacts with living matter, the basic physical effects can be accompanied by biophysical and biochemical phenomena, including cell excitation, electroporation, and electrofusion. To study these phenomena, we developed an experimental system that provides easy sample insertion and removal, protection from airborne particles, observability during the experiment, accurate discharge origin positioning, discharge delivery into the sample either through ...
Source: Bioelectrochemistry - September 10, 2013 Category: Biochemistry Authors: Marjanovič I, Kotnik T Tags: Bioelectrochemistry Source Type: research