Editorial Board
Publication date: May 2021Source: Protein Expression and Purification, Volume 181Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 20, 2021 Category: Biochemistry Source Type: research

Improving SDS-PAGE method for Monoclonal antibodies: The advantages of Tris-Acetate over Tris-Glycine SDS-PAGE system and comparison with CE-SDS method
Publication date: Available online 14 February 2021Source: Protein Expression and PurificationAuthor(s): Rozhan Sonboli, Zeinab Najafi, Nahid Zarezadeh, Mohsen Yazdani, Hossein Behrouz (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 16, 2021 Category: Biochemistry Source Type: research

Expression and characterization of a novel trehalase from Microvirga sp. strain MC18
Publication date: Available online 13 February 2021Source: Protein Expression and PurificationAuthor(s): Chaonan Dong, Qiwen Fan, Xu Li, Yan Huang, Jian Han, Xiaodong Fang, Minghui Huan, Xianfeng Ye, Zhoukun Li, Zhongli Cui (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 15, 2021 Category: Biochemistry Source Type: research

Functional expression and purification of recombinant full-length human ATG7 protein with HIV-1 Tat peptide in Escherichia coli
Publication date: Available online 13 February 2021Source: Protein Expression and PurificationAuthor(s): Guofu Dong, Xi Zhao, Junwang Guo, Lei Ma, Hongmei Zhou, Qi Liu, Xuelong Zhao, Changzhen Wang, Ke Wu (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 15, 2021 Category: Biochemistry Source Type: research

Removing a single-arm species by Fibro PrismA in purifying an asymmetric IgG-like bispecific antibody
Publication date: Available online 13 February 2021Source: Protein Expression and PurificationAuthor(s): Ting Zhang, Yan Wan, Ying Wang, Yifeng Li (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 15, 2021 Category: Biochemistry Source Type: research

Identification of chicken-derived scFv against N-glycolylneuraminic acid retrieved from an immune library by phage display
Publication date: Available online 11 February 2021Source: Protein Expression and PurificationAuthor(s): Han Wang, Zong-Cheng Wu, Pan Hu, Hong-Lin Ren, Yan-Song Li, Yu Zheng, Cong Wang, Zeng-Shan Liu, Shi-Ying Lu (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 13, 2021 Category: Biochemistry Source Type: research

A Custom ÄKTA Avant Configuration Enabling Automated Parallel Protein Purification over a Range of Process Scales
Publication date: Available online 11 February 2021Source: Protein Expression and PurificationAuthor(s): Cristopher Hollander, Shana C. Walrond, Christopher Connelly, Lisa Megson, Elia Bove, Thomas McDonagh (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 13, 2021 Category: Biochemistry Source Type: research

Expression and characterization of a thermotolerant and pH-stable hyaluronate lyase from Thermasporomyces composti DSM22891
Publication date: Available online 7 February 2021Source: Protein Expression and PurificationAuthor(s): Xiaoyi Wang, Shilong Zhang, Hao Wu, Yujiao Li, Wengong Yu, Feng Han (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 9, 2021 Category: Biochemistry Source Type: research

Purification, secondary structure and antioxidant activity of metallothionein zinc-binding proteins from Arca subcrenata
Publication date: Available online 6 February 2021Source: Protein Expression and PurificationAuthor(s): Chunying Meng, Kuiwu wang, Xiaojun zhang, Xinyue Zhu (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 7, 2021 Category: Biochemistry Source Type: research

Overexpression and purification of a toxic peptide LaIT2 from Japanese scorpion, Liocheles australasiae
Publication date: Available online 3 February 2021Source: Protein Expression and PurificationAuthor(s): Maiki Tamura, Chiharu Tatsushiro, Eugene Hayato Morita, Shinya Ohki (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 4, 2021 Category: Biochemistry Source Type: research

An improved production and purification protocol for recombinant soluble human fibroblast activation protein alpha
Publication date: Available online 30 January 2021Source: Protein Expression and PurificationAuthor(s): Cecy R. Xi, Arianna Di Fazio, Naveed Ahmed Nadvi, Michelle Sui Wen Xiang, Hui Emma Zhang, Chandrika Deshpande, Yiqian Chen, Mehdi Sharifi Tabar, Xin Maggie Wang, Charles G. Bailey, Geoffrey W. McCaughan, W Bret Church, Mark D. Gorrell (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 31, 2021 Category: Biochemistry Source Type: research

High Level Stable Expression of Recombinant HIV gp120 in Glutamine Synthetase Gene Deficient HEK293T Cells
Publication date: Available online 30 January 2021Source: Protein Expression and PurificationAuthor(s): Zhongcheng Zou, Ruipeng Wang, Eden P. Go, Heather Desaire, Peter D. Sun (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 31, 2021 Category: Biochemistry Source Type: research

Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2
Publication date: Available online 30 January 2021Source: Protein Expression and PurificationAuthor(s): Shuwei Hu, Xiaoyu Chen, Mengying Zhu, Huifang Hu, Chen Zhou, Li Shi, Naixia Zhang (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 31, 2021 Category: Biochemistry Source Type: research

Characterisation and comparative analysis of hydrophobin isolated from Pleurotus floridanus (PfH)
Publication date: Available online 29 January 2021Source: Protein Expression and PurificationAuthor(s): C.M. Rafeeq, A.B. Vaishnav, P.P. Manzur Ali (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 30, 2021 Category: Biochemistry Source Type: research

Sampling the cultivation parameter space for the bacterial production of TLR1 intracellular domain reveals the multiple optima
Publication date: Available online 29 January 2021Source: Protein Expression and PurificationAuthor(s): Marina V. Goncharuk, Vladislav A. Lushpa, Sergey A. Goncharuk, Alexander S. Arseniev, Konstantin S. Mineev (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 30, 2021 Category: Biochemistry Source Type: research

Medium Optimization for high yield production of human serum albumin in Pichia pastoris and its efficient purification
Publication date: Available online 26 January 2021Source: Protein Expression and PurificationAuthor(s): Wen Zhu, Renren Xu, Guihua Gong, Lei Xu, Youjia Hu, Liping Xie (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 27, 2021 Category: Biochemistry Source Type: research

PiP2 favors an α-helical structure of non-recombinant Hsp12 of Saccharomyces cerevisiae
Publication date: Available online 22 January 2021Source: Protein Expression and PurificationAuthor(s): Antoine Léger, Mehdi Azouz, Sophie Lecomte, François Dole, Agnès Hocquellet, Stéphane Chaignepain, Charlotte Cabanne (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 23, 2021 Category: Biochemistry Source Type: research

Editorial Board
Publication date: April 2021Source: Protein Expression and Purification, Volume 180Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 23, 2021 Category: Biochemistry Source Type: research

Pseudomonas aeruginosa recombinant L-asparaginase: large scale production, purification, and cytotoxicity on THP-1, MDA-MB-231, A549, Caco2 and HCT-116 cell lines
Publication date: Available online 11 January 2021Source: Protein Expression and PurificationAuthor(s): Hesham Saeed, Asmaa Hemida, Manal Abdel-Fattah, Ahmad Eldoksh, Manal Shalaby, Hisham Nematalla, Nefertiti El-Nikhely (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 12, 2021 Category: Biochemistry Source Type: research

Application of pH-salt dual gradient elution in purifying a WuXiBody-based bispecific antibody by MMC ImpRes mixed-mode chromatography
Publication date: Available online 9 January 2021Source: Protein Expression and PurificationAuthor(s): Yan Wan, Yumeng Wang, Ting Zhang, Shengwei Zhang, Ying Wang, Yifeng Li (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 10, 2021 Category: Biochemistry Source Type: research

Purification and characterization of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry
Publication date: April 2021Source: Protein Expression and Purification, Volume 180Author(s): Sumeyra Gurkok, Murat Ozdal (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 10, 2021 Category: Biochemistry Source Type: research

Expression, purification and characterization of the RhoA-binding domain of human SHIP2 in E.coli
Publication date: Available online 6 January 2021Source: Protein Expression and PurificationAuthor(s): Heng Zhou, Xiali Yue, Zi Wang, Shuangli Li, Jiang Zhu, Yunhuang Yang, Maili Liu (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 7, 2021 Category: Biochemistry Source Type: research

Construction, expression and purification of a novel CadF–based multiepitope antigen and its immunogenic polyclonal antibody specific to Campylobacter jejuni and Campylobacter coli
Publication date: Available online 5 January 2021Source: Protein Expression and PurificationAuthor(s): Pattarapong Wenbap, Yortyot Seetang–Nun, Taradon Luangtongkum, Pongsak Khunrae, Pravate Tuitemwong, Triwit Rattanarojpong (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 6, 2021 Category: Biochemistry Source Type: research

Purification and characterisation of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry
Publication date: Available online 5 January 2021Source: Protein Expression and PurificationAuthor(s): Sumeyra Gurkok, Murat Ozdal (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 6, 2021 Category: Biochemistry Source Type: research

Editorial Board
Publication date: March 2021Source: Protein Expression and Purification, Volume 179Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - December 24, 2020 Category: Biochemistry Source Type: research

Cloning, Expression and Characterization of P1 and NIa proteases from Banana Bract Mosaic Virus (BBrMV)
Publication date: Available online 18 December 2020Source: Protein Expression and PurificationAuthor(s): Atul B. Patil, Vijayendra S. Dalvi, Abdul Azeez, Bal Krishna, Akhilesh A. Mishra, Prafullachandra V. Sane (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - December 19, 2020 Category: Biochemistry Source Type: research

Efficient improvement of surface displayed lipase from Rhizomucor miehei in PichiaPink™ protease-deficient system
Publication date: April 2021Source: Protein Expression and Purification, Volume 180Author(s): Zhansheng Li, Yangli Miao, Jiaming Yang, Fengguang Zhao, Ying Lin, Shuangyan Han (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - December 19, 2020 Category: Biochemistry Source Type: research

O-acetylhomoserine sulfhydrylase from Clostridium novyi. Cloning, expression of the gene and characterization of the enzyme
Publication date: Available online 16 December 2020Source: Protein Expression and PurificationAuthor(s): Vitalia V. Kulikova, Natalya V. Anufrieva, Mikhail I. Kotlov, Elena A. Morozova, Vasiliy S. Koval, Yury F. Belyi, Svetlana V. Revtovich, Tatyana V. Demidkina (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - December 17, 2020 Category: Biochemistry Source Type: research

Efficient soluble production of folded cat allergen Fel d 1 in Escherichia coli
Publication date: Available online 15 December 2020Source: Protein Expression and PurificationAuthor(s): Chi Zhang, Rosario Recacha, Lloyd W. Ruddock, Antti Moilanen (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - December 16, 2020 Category: Biochemistry Source Type: research

Soluble expression, purification, and secondary structure determination of human PDX1 transcription factor
Publication date: Available online 11 December 2020Source: Protein Expression and PurificationAuthor(s): Gloria Narayan, Pradeep Kumar Sundaravadivelu, Akriti Agrawal, Ranadeep Gogoi, Shirisha Nagotu, Rajkumar P. Thummer (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - December 13, 2020 Category: Biochemistry Source Type: research

Combining the advantages of prokaryotic expression and T7 phage display systems to obtain antigens for antibody preparation
Publication date: Available online 11 December 2020Source: Protein Expression and PurificationAuthor(s): Jingrui Huo, Guoan Zhang, Lei Wang, Wenjie Sun, Li Jia, Xiaohui Yang, Yingfu Liu (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - December 13, 2020 Category: Biochemistry Source Type: research

Comparison of three palm tree peroxidases expressed by Escherichia coli: uniqueness of African oil palm peroxidase
Publication date: Available online 7 December 2020Source: Protein Expression and PurificationAuthor(s): Meile Yuan, Hongwei Zhao, Qian Huang, Xianhua Liu, Yanyu Zhou, Xiaoping Diao, Qing X. Li (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - December 8, 2020 Category: Biochemistry Source Type: research

Optimization of culture conditions for high-level expression of soluble and active tumor necrosis factor-α in E. coli
Publication date: Available online 5 December 2020Source: Protein Expression and PurificationAuthor(s): Shadi Damough, Masoumeh Sabzalinezhad, Yeganeh Talebkhan, Leila Nematollahi, Elham Bayat, Fatemeh Torkashvand, Ahmad Adeli, Hoda Jahandar, Farzaneh Barkhordari, Fereidoun Mahboudi (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - December 7, 2020 Category: Biochemistry Source Type: research

Efficient Improvement of Surface Displayed Lipase from Rhizomucor miehei in PichiaPinkTM Protease-deficient System
Publication date: Available online 2 December 2020Source: Protein Expression and PurificationAuthor(s): Zhansheng Li, Yangli Miao, Jiaming Yang, Fengguang Zhao, Ying Lin, Shuangyan Han (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - December 3, 2020 Category: Biochemistry Source Type: research

Expression, purification and characterization of TMCO1 for structural studies
Publication date: Available online 27 November 2020Source: Protein Expression and PurificationAuthor(s): Ningning Zhang, Meng Tang, Maorong Wen, Yu Cao, Bo OuYang (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 28, 2020 Category: Biochemistry Source Type: research

Effect of Y50H and S187G substitutions on thermostability and exonuclease activity of TK1646 from Thermococcus kodakarensis
Publication date: Available online 26 November 2020Source: Protein Expression and PurificationAuthor(s): Muhammad Sulaiman Saeed, Masood Ahmed Siddiqui, Naeem Rashid (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 27, 2020 Category: Biochemistry Source Type: research

Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays
Publication date: Available online 25 November 2020Source: Protein Expression and PurificationAuthor(s): Jennifer Mehalko, Matthew Drew, Kelly Snead, John-Paul Denson, Vanessa Wall, Troy Taylor, Kaitlyn Sadtler, Simon Messing, William Gillette, Dominic Esposito (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 26, 2020 Category: Biochemistry Source Type: research

Recombinant expression of the precursor of rat lung surfactant protein B in Escherichia coli and its antibacterial mechanism
Publication date: Available online 25 November 2020Source: Protein Expression and PurificationAuthor(s): Wulong Cao, Qin Liu, Ting Wang, Qiuhan Zhang, Fu Cheng, Yishan Tang, Chenchen Mei, Fang Wen, Wanneng Wang (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 26, 2020 Category: Biochemistry Source Type: research

Cloning, purification and biochemical characterization of two glutathione S-transferase isoforms from Rutilus frisii kutum
Publication date: Available online 25 November 2020Source: Protein Expression and PurificationAuthor(s): Salman Ahmadi, Hossein Ghafouri, Sara Tarazi, Sajjad Sarikhan, Omid Saberi Kh (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 26, 2020 Category: Biochemistry Source Type: research

Msx1 cooperates with Runx1 for inhibiting myoblast differentiation
Publication date: Available online 24 November 2020Source: Protein Expression and PurificationAuthor(s): Guoqiang Zhou, Yenan Yang, Xumin Zhang, Jingqiang Wang (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 26, 2020 Category: Biochemistry Source Type: research

Characterization of an acidic pectin methylesterase from Paenibacillus xylanexedens and its application in fruit processing
Publication date: Available online 21 November 2020Source: Protein Expression and PurificationAuthor(s): Lingli Zhong, Xiaowen Wang, Lin Fan, Xianfeng Ye, Zhoukun Li, Zhongli Cui, Yan Huang (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 23, 2020 Category: Biochemistry Source Type: research

Development of a robust crystallization platform for immune receptor TREM2 using a crystallization chaperone strategy
Publication date: Available online 20 November 2020Source: Protein Expression and PurificationAuthor(s): Noel J. Byrne, Amy C. Lee, James Kostas, John C. Reid, Andrea T. Partridge, Sung-Sau So, Joseph E. Cowan, Pravien Abeywickrema, Hua Huang, Matthias Zebisch, John J. Barker, Stephen M. Soisson, Alexei Brooun, Hua-Poo Su (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 21, 2020 Category: Biochemistry Source Type: research

Purification, crystallization, and X-ray diffraction analysis of myocyte enhancer factor 2D and DNA complex
Publication date: Available online 19 November 2020Source: Protein Expression and PurificationAuthor(s): Zhiming Chen, Qianqian Wang, Hao Zhang, Xiaodan Ma, Wenyu Wu, Nuo Cheng, Ji Zhang, Aiwu Zhou, Yuwen Li, Guoyu Meng (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 20, 2020 Category: Biochemistry Source Type: research

Editorial Board
Publication date: February 2021Source: Protein Expression and Purification, Volume 178Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 20, 2020 Category: Biochemistry Source Type: research

Expression, Purification and Functional Identification of the Modified hEGF Protein
Publication date: Available online 14 November 2020Source: Protein Expression and PurificationAuthor(s): Huifang Guo, Fengrui Hu, Kang Li, Zhaohua Zhao, Zhi Li, Xi Xu, Xingchun Gou (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 15, 2020 Category: Biochemistry Source Type: research

Expression, purification and identification of isotope-labeled recombinant cystatin C protein in Escheichia coli intended for absolute quantification using isotope dilution mass spectrometry
Publication date: Available online 3 November 2020Source: Protein Expression and PurificationAuthor(s): Qiaoxuan Zhang, Zhiliang Cai, Haibiao Lin, Liqiao Han, Jun Yan, Jianbing Wang, Peifeng Ke, Junhua Zhuang, Xianzhang Huang (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 4, 2020 Category: Biochemistry Source Type: research

Impact of salt concentration in mobile phase on antibody retention in Protein A, Protein L and KappaSelect affinity chromatography
Publication date: Available online 3 November 2020Source: Protein Expression and PurificationAuthor(s): Ying Wang, Xiujuan Chen, Ying Wang, Yifeng Li (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 4, 2020 Category: Biochemistry Source Type: research

Purification, Crystallization and X-ray analysis of Pf-SCP (Sarcoplasmic Ca-binding Protein), related to storage and transport of calcium in mantle of Pinctada fucata
Publication date: Available online 31 October 2020Source: Protein Expression and PurificationAuthor(s): Lingxiao Zhu, Liying Wang, Akihiro Matsuura, Mimin Zhang, Peng Lu, Kurin Iimura, Koji Nagata, Michio Suzuki (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - November 1, 2020 Category: Biochemistry Source Type: research

Expression and purification of the antimicrobial peptide Bin1b in Escherichia coli tagged with the fusion proteins CusF3H+ and SmbP
Publication date: Available online 28 October 2020Source: Protein Expression and PurificationAuthor(s): Jorge M. Montfort-Gardeazabal, Isaias Balderas-Renteria, Nestor G. Casillas-Vega, Xristo Zarate (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - October 29, 2020 Category: Biochemistry Source Type: research

Corrigendum to ‘Production of active recombinant human aldehyde oxidase (AOX) in the baculovirus expression vector system (BEVS) and deployment in a pre-clinical fraction-of-control AOX compound exposure assay’ [Protein Expr. Purif. 177 (2020) 105749]
Publication date: Available online 27 October 2020Source: Protein Expression and PurificationAuthor(s): Ciarán N. Cronin, JianHua Liu, Nicole Grable, Timothy J. Strelevitz, R. Scott Obach, Anthony Carlo (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - October 28, 2020 Category: Biochemistry Source Type: research