Facile and scalable expression and purification of transcription factor IIH (TFIIH) core complex
Publication date: Available online 27 May 2020Source: Protein Expression and PurificationAuthor(s): Reyes Sanles-Falagan, Biljana Petrovic-Stojanovska, Malcolm F. White (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 29, 2020 Category: Biochemistry Source Type: research

Affimers as an alternative to antibodies for protein biomarker enrichment
Publication date: Available online 24 May 2020Source: Protein Expression and PurificationAuthor(s): Roel Tans, Danique M.H. van Rijswijck, Alex Davidson, Ryan Hannam, Bryon Ricketts, Cees J. Tack, Hans J.C.T. Wessels, Jolein Gloerich, Alain J. van Gool (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 26, 2020 Category: Biochemistry Source Type: research

Expression and purification of the human tumor suppressor protein RNASET2 in CHO–S cells
Publication date: Available online 23 May 2020Source: Protein Expression and PurificationAuthor(s): Elena Rosini, Annarosaria De Vito, Federica Ponti, Debora Scaldaferri, Loredano Pollegioni, Francesco Acquati (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 24, 2020 Category: Biochemistry Source Type: research

Expression, purification and characterization of a cold-adapted dextranase from marine bacteria and its ability to remove dental plaque
Publication date: Available online 21 May 2020Source: Protein Expression and PurificationAuthor(s): Tian Deng, Yanli Feng, Linxiang Xu, Xiaopeng Tian, Xiaohua Lai, Mingsheng Lyu, Shujun Wang (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 23, 2020 Category: Biochemistry Source Type: research

High cell-density fermentation, expression and purification of bacteriophage lysin TSPphg, a thermostable antimicrobial protein from extremophilic Thermus bacteriophage TSP4
Publication date: Available online 20 May 2020Source: Protein Expression and PurificationAuthor(s): Feng Wang, Guanling Zhang, Jiani Peng, Xinyu Ji, Jun Hai, Xianyu Deng, Lianbing Lin (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 22, 2020 Category: Biochemistry Source Type: research

Editorial Board
Publication date: September 2020Source: Protein Expression and Purification, Volume 173Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 17, 2020 Category: Biochemistry Source Type: research

Cloning and characterization of the Bambusa oldhamii BoMDH-encoded malate dehydrogenase
Publication date: Available online 13 May 2020Source: Protein Expression and PurificationAuthor(s): Che-Jen Hsiao, Chun-Yen Hsieh, Lu-Sheng Hsieh (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 14, 2020 Category: Biochemistry Source Type: research

Expression and purification of soluble recombinant SapM from Mycobacterium tuberculosis
Publication date: Available online 6 May 2020Source: Protein Expression and PurificationAuthor(s): Paulina Fernandez-Soto, Jennifer S. Cavet, Lydia Tabernero (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 8, 2020 Category: Biochemistry Source Type: research

Affinity tag effect on the salt rejection potential of Halomonas elongata aquaporin incorporated in thin film nanocomposite membrane
Publication date: Available online 5 May 2020Source: Protein Expression and PurificationAuthor(s): Hilal Yılmaz, Fatma İnci Özdemir, Pınar Ergenekon, Melek Özkan (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 6, 2020 Category: Biochemistry Source Type: research

Purification, biochemical characterization and DNA protection against oxidative damage of a novel recombinant superoxide dismutase from psychrophilic bacterium Halomonas sp. ANT108
Publication date: Available online 5 May 2020Source: Protein Expression and PurificationAuthor(s): Quanfu Wang, Ping Nie, Yanhua Hou, Yatong Wang (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 6, 2020 Category: Biochemistry Source Type: research

A simplified method for producing laboratory grade recombinant TEV protease from E. coli
Publication date: Available online 5 May 2020Source: Protein Expression and PurificationAuthor(s): Jordan Brungardt, Revathi Govind, Harold N. Trick (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 6, 2020 Category: Biochemistry Source Type: research

Editorial Board
Publication date: August 2020Source: Protein Expression and Purification, Volume 172Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 5, 2020 Category: Biochemistry Source Type: research

High-yield production in E. coli and characterization of full-length functional p13II protein from human T-cell leukemia virus type 1
Publication date: Available online 30 April 2020Source: Protein Expression and PurificationAuthor(s): Elka R. Georgieva, Peter P. Borbat, Christina Fanouraki, Jack H. Freed (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 2, 2020 Category: Biochemistry Source Type: research

Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition
Publication date: Available online 29 April 2020Source: Protein Expression and PurificationAuthor(s): Mohammad Rahmati-Yamchi, Mohammad Karimi Babaahmadi, Seyed Abolghasem Mohammadi, Manoochehr Makvandi, Morteza Mamoueie, David Wood (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 30, 2020 Category: Biochemistry Source Type: research

Fermentation, purification and immunogenicity of a recombinant tumor multi-epitope vaccine, VBP3
Publication date: Available online 28 April 2020Source: Protein Expression and PurificationAuthor(s): Ligang Zhang, Xi Chen, Yanrui Deng, Chengcheng Jiang, Ning Deng (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 29, 2020 Category: Biochemistry Source Type: research

Robust and facile purification of full-length, untagged human Nedd4 as a recombinant protein from Escherichia coli
Publication date: Available online 23 April 2020Source: Protein Expression and PurificationAuthor(s): A. Katherine Hatstat, Dewey G. McCafferty (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 25, 2020 Category: Biochemistry Source Type: research

A potential downstream platform approach for WuXiBody-based IgG-like bispecific antibodies
Publication date: Available online 23 April 2020Source: Protein Expression and PurificationAuthor(s): Gaili Guo, Jinyu Han, Ying Wang, Yifeng Li (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 25, 2020 Category: Biochemistry Source Type: research

Expression, purification and oligomerization of the S-adenosylmethionine transporter
Publication date: Available online 23 April 2020Source: Protein Expression and PurificationAuthor(s): Dandan Wang, Meizi Liu, Xuemiao Li, Xiaoqiang Wang, Yuequan Shen (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 25, 2020 Category: Biochemistry Source Type: research

Non-chromatographic purification of thermostable endoglucanase from Thermotoga maritima by fusion with a hydrophobic elastin-like polypeptide
Publication date: Available online 21 April 2020Source: Protein Expression and PurificationAuthor(s): Shanshan Wang, Rui Lin, Yanyan Ren, Tao Zhang, Hongzhao Lu, Ling Wang, Daidi Fan (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 23, 2020 Category: Biochemistry Source Type: research

Refolding, characterization, and dye decolorization ability of a highly thermostable laccase from Geobacillus sp. JS12
Publication date: Available online 18 April 2020Source: Protein Expression and PurificationAuthor(s): Sung-Jong Jeon, Jong-Hun Park (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 20, 2020 Category: Biochemistry Source Type: research

Recombinant expression of nanobodies and nanobody-derived immunoreagents
Publication date: Available online 11 April 2020Source: Protein Expression and PurificationAuthor(s): Ario de Marco (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 13, 2020 Category: Biochemistry Source Type: research

Expression and purification of the heme exporter FLVCR1a
Publication date: Available online 8 April 2020Source: Protein Expression and PurificationAuthor(s): Deborah Chiabrando, Luigi Scietti, Adriana Georgiana Prajica, Francesca Bertino, Emanuela Tolosano, Francesca Magnani (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 10, 2020 Category: Biochemistry Source Type: research

Editorial Board
Publication date: July 2020Source: Protein Expression and Purification, Volume 171Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 10, 2020 Category: Biochemistry Source Type: research

Peptidyl-prolyl isomerase and the biological activities of recombinant protein cyclophilin from Pyropia yezoensis (PyCyp)
Publication date: Available online 7 April 2020Source: Protein Expression and PurificationAuthor(s): Selvakumari Ulagesan, Jeong-Wook Choi, Taek-Jeong Nam, Youn-Hee Choi (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 8, 2020 Category: Biochemistry Source Type: research

Removing half antibody byproduct by protein A chromatography during the purification of a bispecific antibody
Publication date: Available online 5 April 2020Source: Protein Expression and PurificationAuthor(s): Xiujuan Chen, Ying Wang, Yifeng Li (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 7, 2020 Category: Biochemistry Source Type: research

Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis
Publication date: Available online 4 April 2020Source: Protein Expression and PurificationAuthor(s): Wei Hang, Asadullah Gujar, Hongjiang Zhang, Wenxin Xu, Chunchao Zhao, Xiaoli Zhu, Jinai Xue, Chunhui Zhang, Chunli Ji, Song Qin, Runzhi Li, Hongli Cui (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 5, 2020 Category: Biochemistry Source Type: research

Recombinant protein production and purification of SiiD, SiiE and SiiF - Components of the SPI4-encoded type I secretion system from Salmonella Typhimurium
Publication date: Available online 3 April 2020Source: Protein Expression and PurificationAuthor(s): Stefan Klingl, Sina Kordes, Benedikt Schmid, Roman G. Gerlach, Michael Hensel, Yves A. Muller (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - April 4, 2020 Category: Biochemistry Source Type: research

Cloning, expression and purification of the low-complexity region of RanBP9 protein
Publication date: Available online 23 March 2020Source: Protein Expression and PurificationAuthor(s): Shailendra Dhakal, Krishna Sapkota, Faqing Huang, Vijayaraghavan Rangachari (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 24, 2020 Category: Biochemistry Source Type: research

A novel recombinant expression and purification approach for the full-length anti-apoptotic membrane protein Bcl-2
Publication date: Available online 22 March 2020Source: Protein Expression and PurificationAuthor(s): Jörgen Ådén, Ameeq Ul Mushtaq, Artur Dingeldein, Marcus Wallgren, Gerhard Gröbner (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 23, 2020 Category: Biochemistry Source Type: research

Efficient preparation of human and mouse CD1d proteins using silkworm baculovirus expression system
Publication date: Available online 22 March 2020Source: Protein Expression and PurificationAuthor(s): Hiroki Kusaka, Shunsuke Kita, Takashi Tadokoro, Kouki Yoshida, Yoshiyuki Kasai, Harumi Niiyama, Yukari Fujimoto, Shinya Hanashima, Michio Murata, Shigeru Sugiyama, Toyoyuki Ose, Kimiko Kuroki, Katsumi Maenaka (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 23, 2020 Category: Biochemistry Source Type: research

Selection, preparation and characterization of scFv against human lipocalin 6 by phage display technology
Publication date: Available online 20 March 2020Source: Protein Expression and PurificationAuthor(s): Jiong Chen, Yue Zhao, Wei Feng (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 21, 2020 Category: Biochemistry Source Type: research

The expression, purification, and substrate analysis of matrix metalloproteinases in Drosophila melanogaster
Publication date: Available online 19 March 2020Source: Protein Expression and PurificationAuthor(s): Di Wen, Zhi Chen, Zeyan Zhang, Qiangqiang Jia (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 21, 2020 Category: Biochemistry Source Type: research

Rapid purification method for human SFPQ by implementing zinc-induced polymerization
Publication date: Available online 17 March 2020Source: Protein Expression and PurificationAuthor(s): Yee Wa Lim, Mihwa Lee (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 18, 2020 Category: Biochemistry Source Type: research

Production of bioactive human IFN-γ protein by agroinfiltration in tobacco
Publication date: Available online 14 March 2020Source: Protein Expression and PurificationAuthor(s): Reza Heidari-Japelaghi, Mostafa Valizadeh, Raheem Haddad, Ebrahim Dorani-Uliaie, Mokhtar Jalali-Javaran (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 16, 2020 Category: Biochemistry Source Type: research

The open reading frame 02797 from Candida tropicalis encodes a novel NADH-dependent aldehyde reductase
Publication date: Available online 12 March 2020Source: Protein Expression and PurificationAuthor(s): Xiangdong Hu, Xuebing Han, Lan Wu, Hanyu Wang, Yidan Ouyang, Qian Li, Xiaolin Kuang, Quanju Xiang, Xiumei Yu, Xi Li, Yunfu Gu, Ke Zhao, Qiang Chen, Menggen Ma (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 13, 2020 Category: Biochemistry Source Type: research

Editorial Board
Publication date: June 2020Source: Protein Expression and Purification, Volume 170Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 11, 2020 Category: Biochemistry Source Type: research

Separating antibody species containing one and two kappa light chain constant region by KappaSelect affinity chromatography
Publication date: Available online 7 March 2020Source: Protein Expression and PurificationAuthor(s): Taotao Qin, Ying Wang, Yifeng Li (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 8, 2020 Category: Biochemistry Source Type: research

Purification of native CCL7 and its functional interaction with selected chemokine receptors
Publication date: Available online 5 March 2020Source: Protein Expression and PurificationAuthor(s): Marina V. Goncharuk, Debarati Roy, Maxim A. Dubinnyi, Kirill D. Nadezhdin, Ashish Srivastava, Mithu Baidya, Hemlata Dwivedi-Agnihotri, Alexander S. Arseniev, Arun K. Shukla (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - March 7, 2020 Category: Biochemistry Source Type: research

Comparison of physicochemical properties of recombinant buckwheat trypsin inhibitor (rBTI) and soybean trypsin inhibitor (SBTI)
Publication date: Available online 28 February 2020Source: Protein Expression and PurificationAuthor(s): Chen Li, Wenjie Li, Yi Zhang, Benjamin Kofi Simpson (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 29, 2020 Category: Biochemistry Source Type: research

Expression, purification, and characterization of recombinant apoPholasin
Publication date: Available online 28 February 2020Source: Protein Expression and PurificationAuthor(s): Satoshi Inouye, Yuiko Sahara-Miura, Mitsuhiro Nakamura, Takamitsu Hosoya (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 29, 2020 Category: Biochemistry Source Type: research

Expression and characterization of a chitinase from Serratia marcescens
Publication date: Available online 23 February 2020Source: Protein Expression and PurificationAuthor(s): Jincheng Li, Jiamin Zheng, Yanhui Liang, Renxiang Yan, Xinqi Xu, Juan LinAbstractA chitinase gene from Serratia marcescens was cloned and expressed in Escherichia coli and the properties of recombinant chitinase rCHI-2 were characterized. The optimum catalytic pH of rCHI-2 was 6.0. It was stable in the pH range of 6.0–9.0 and could maintain more than 90% of its relative enzyme activity after incubation at 37 °C for 1 h. The optimum catalytic temperature of the enzyme was 55 °C and 85% of enzyme activ...
Source: Protein Expression and Purification - February 24, 2020 Category: Biochemistry Source Type: research

A new insight into purification of polyphenol oxidase and inhibition effect of curcumin and quercetin on potato polyphenol oxidase
Publication date: Available online 22 February 2020Source: Protein Expression and PurificationAuthor(s): Mine AksoyAbstractIn the literature, the polyphenol oxidase (PPO) enzyme has been purified a many times via Sepharose 4B-l-tyrosine-p-aminobenzoic acid affinity column. In order to study PPO purification efficiency, 2-aminophenol and 4-aminophenol were applied as a spacer arm to CNBr-activated Sepharose 4B. The effects of the spacer arm on specific activity, purification fold, and electrophoretic properties were investigated. The best performance with 11.7-fold purification and 23951 U/mg protein specific activity was a...
Source: Protein Expression and Purification - February 24, 2020 Category: Biochemistry Source Type: research

Optimization of production conditions of rice α-galactosidase II displayed on yeast cell surface
Publication date: Available online 21 February 2020Source: Protein Expression and PurificationAuthor(s): Mosi Dong, Yun Gong, Jia Guo, Jing Ma, Suhong Li, Tuoping LiAbstractThe yeast surface displayed rice α-galactosidase II (YSD rice α-Gal II) was generated with the pYD1 vector. The expression and cultural conditions for the improvement of production of YSD rice α-Gal II were optimized. The results showed that several induction factors, which were the initial cell density, inoculation ratio, galactose (inducer) concentration, induction time and temperature, determined the activity and expression efficien...
Source: Protein Expression and Purification - February 23, 2020 Category: Biochemistry Source Type: research

An improved method for the expression and purification of porcine dihydropyrimidine dehydrogenase
Publication date: Available online 21 February 2020Source: Protein Expression and PurificationAuthor(s): Brett A. Beaupre, Joseph V. Roman, Graham R. MoranAbstractDihydropyrimidine dehydrogenase (DPD) catalyzes the reduction of uracil and thymine bases with electrons derived from NADPH. The mammalian DPD enzyme is a functional homodimer and has an elaborate cofactor arrangement. Two flavin cofactors (FAD and FMN) reside in two active site cavities that are separated by around 60 Å. The flavins are apparently bridged by four Fe4S4 clusters, two of which are provided by the partner protomer of the dimer. The study of...
Source: Protein Expression and Purification - February 23, 2020 Category: Biochemistry Source Type: research

Editorial Board
Publication date: May 2020Source: Protein Expression and Purification, Volume 169Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 20, 2020 Category: Biochemistry Source Type: research

Starch-degrading enzymes from the brown-rot fungus Fomitopsis palustris
Publication date: Available online 16 February 2020Source: Protein Expression and PurificationAuthor(s): Yuki Tanaka, Naotake Konno, Tomohiro Suzuki, Naoto HabuAbstractBrown-rot fungi preferentially degrade softwood and cause severe breakdown of wooden structures. At the initial stage of the brown-rot decay, penetrating hyphae of the fungi are observed in ray parenchyma. Since starch grains are known to be present in the ray parenchyma of sapwood, investigation of the functions and roles of the starch-degrading enzymes is important to understand the initial stage of brown-rot decay. We purified and characterized two starch...
Source: Protein Expression and Purification - February 17, 2020 Category: Biochemistry Source Type: research

Affinity purification of Car9-tagged proteins on silica-derivatized spin columns and 96-well plates
Publication date: Available online 14 February 2020Source: Protein Expression and PurificationAuthor(s): Meng Xu, Matthew J. Bailey, Jennifer Look, Francois BaneyxAbstractThe Car9 affinity tag is a dodecameric silica-binding peptide that can be fused to the N- and C-termini of proteins of interest to enable their rapid and inexpensive purification on underivatized silica in a process that typically relies on l-lysine as an eluent. Here, we show that silica paper spin columns and borosilicate multi-well plates used for plasmid DNA purification are suitable for recovering Car9-tagged proteins with high purity in a workflow c...
Source: Protein Expression and Purification - February 15, 2020 Category: Biochemistry Source Type: research

Nanobody production can be simplified by direct secretion from Escherichia coli
Publication date: Available online 13 February 2020Source: Protein Expression and PurificationAuthor(s): Takayuki Iwaki, Kimiko Hara, Kazuo UmemuraAbstractIt is well known that camelids (camels and llamas) have fully functional antibodies with only a heavy chain consisting of a single variable domain and two constant domains. This single variable domain is called a “nanobody” and many nanobodies are synthesized in the cytosol of Escherichia coli, however, most of the nanobodies become inclusion bodies without tags to enhance their solubility. We generated a vector system to enable the secretary expression of na...
Source: Protein Expression and Purification - February 14, 2020 Category: Biochemistry Source Type: research

Initial development of biosimilar immune checkpoint blockers using HEK293 cells
Publication date: June 2020Source: Protein Expression and Purification, Volume 170Author(s): Michael Bernardes Ramos, Anna Erika Vieira de Araújo, Cristiane Pinheiro Pestana, Ana Paula Dinis Ano Bom, Renata Chagas Bastos, Aline de Almeida Oliveira, Patrícia Cristina da Costa Neves, Haroldo Cid da Silva JuniorAbstractAntibodies that block interaction of immune checkpoint receptors with its ligands have revolutionized the treatment of several cancers. Despite the success of this approach, the high cost has been restricted the use of this class of drugs. In this context, the development of biosimilar can be an i...
Source: Protein Expression and Purification - February 14, 2020 Category: Biochemistry Source Type: research

Expression and purification of recombinant human serpin B1 yields novel molecules with altered protease inhibitory activities: Functional implications
Publication date: Available online 7 February 2020Source: Protein Expression and PurificationAuthor(s): Philip A. PembertonAbstractSerpin B1 regulates the innate immune system by inhibiting serine and cysteine proteases that control programmed cell death and proliferation pathways. To provide recombinant human proteins for in vitro and in vivo studies we expressed and purified wild-type human serpin B1 and a C344A variant in the yeast S. cerevisiae. Both proteins expressed well and inhibited elastase and chymotrypsin. However, purification of wild-type serpin B1 in the absence of a reducing agent resulted in the specific l...
Source: Protein Expression and Purification - February 8, 2020 Category: Biochemistry Source Type: research