• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

N-Terminomic Changes of Neurons During Excitotoxicity Reveal Proteolytic Events Associated with Synaptic Dysfunctions and Potential Targets for Neuroprotection
Mol Cell Proteomics. 2023 Apr 6:100543. doi: 10.1016/j.mcpro.2023.100543. Online ahead of print.ABSTRACTExcitotoxicity, a neuronal death process in neurological disorders such as stroke, is initiated by over-stimulation of ionotropic glutamate receptors. Although dysregulation of proteolytic signaling networks is critical for excitotoxicity, the identity of affected proteins and mechanisms by which they induce neuronal cell death remain unclear. To address this, we used quantitative N-terminomics to identify proteins modified by proteolysis in neurons undergoing excitotoxic cell death. We found that most proteolytically pr...
Source: Molecular and Cellular Proteomics : MCP - April 8, 2023 Category: Molecular Biology Authors: S Sadia Ameen Nane Griem-Krey Antoine Dufour M Iqbal Hossain Ashfaqul Hoque Sharelle Sturgeon Harshal Nandurkar Dominik F Draxler Robert L Medcalf Mohd Aizuddin Kamaruddin Isabelle S Lucet Michael G Leeming Dazhi Liu Amardeep Dhillon Jet Phey Lim Faiza Ba Source Type: research

Phosphoproteome Analysis Identifies a Synaptotagmin-1-associated complex involved in Ischemic Neuron Injury
Mol Cell Proteomics. 2022 Mar 4:100222. doi: 10.1016/j.mcpro.2022.100222. Online ahead of print.ABSTRACTCerebral stroke is one of the leading causes of death in adults worldwide. However, the molecular mechanisms of stroke-induced neuron injury are not fully understood. Here, we obtained phosphoproteomic and proteomic profiles of the acute ischemic hippocampus by LC-MS/MS analysis. Quantitative phosphoproteomic analyses revealed that the dysregulated phosphoproteins were involved in synaptic components and neurotransmission. We further demonstrated that phosphorylation of Synaptotagmin-1 (Syt1) at the Thr112 site in cultur...
Source: Molecular and Cellular Proteomics : MCP - March 8, 2022 Category: Molecular Biology Authors: Wei Jiang Pei Zhang Peng Yang Na Kang Junqiang Liu Aihemaiti Yilixiati Haijun Tu Source Type: research

A mouse brain-based multi-omics integrative approach reveals potential blood biomarkers for ischemic stroke.
Abstract Stroke remains a leading cause of death and disability worldwide. Despite continuous advances, the identification of key molecular signatures in the hyper-acute phase of ischemic stroke is still a primary interest for translational research on stroke diagnosis, prognosis, and treatment. Data integration from high-throughput -omics techniques has become crucial to unraveling key interactions among different molecular elements in complex biological contexts, such as ischemic stroke. Thus, we used advanced data integration methods for a multi-level joint analysis of transcriptomics and proteomics datasets ob...
Source: Molecular and Cellular Proteomics : MCP - August 30, 2020 Category: Molecular Biology Authors: Simats A, Ramiro L, García-Berrocoso T, Briansó F, Gonzalo R, Martín L, Sabé A, Gill N, Penalba A, Colome N, Sánchez A, Canals F, Bustamante A, Rosell A, Montaner J Tags: Mol Cell Proteomics Source Type: research

Single Cell Immuno-laser Microdissection Coupled to Label-free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia.
In this study, post-mortem brain slices from ischemic stroke patients were obtained corresponding to infarcted (IC) and contralateral (CL) areas. By means of laser microdissection, neurons and blood brain barrier structures (BBB) were isolated and analyzed using label-free quantification. MS data are available via ProteomeXchange with identifier PXD003519. Ninety proteins were identified only in neurons, 260 proteins only in the BBB and 261 proteins in both cell types. Bioinformatics analyses revealed that repair processes, mainly related to synaptic plasticity, are outlined in microdissected neurons, with non-exclusive im...
Source: Molecular and Cellular Proteomics : MCP - November 13, 2017 Category: Molecular Biology Authors: García-Berrocoso T, Llombart V, Colàs-Campàs L, Hainard A, Licker V, Penalba A, Ramiro L, Simats A, Bustamante A, Martínez-Saez E, Canals F, Sanchez JC, Montaner J Tags: Mol Cell Proteomics Source Type: research